Direct injection versus liquid-liquid extraction for plasma sample analysis by high performance liquid chromatography with tandem mass spectrometry
| العنوان: | Direct injection versus liquid-liquid extraction for plasma sample analysis by high performance liquid chromatography with tandem mass spectrometry |
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| المؤلفون: | Xiaohui Jiang, Mark L. Powell, Mike Huang, Mohammed Jemal, Yan Mao |
| المصدر: | Rapid Communications in Mass Spectrometry. 13:2125-2132 |
| بيانات النشر: | Wiley, 1999. |
| سنة النشر: | 1999 |
| مصطلحات موضوعية: | Quality Control, Accuracy and precision, Chromatography, Organic Chemistry, Extraction (chemistry), Analytical chemistry, Mass spectrometry, Tandem mass spectrometry, High-performance liquid chromatography, Mass Spectrometry, Analytical Chemistry, Plasma, chemistry.chemical_compound, chemistry, Liquid–liquid extraction, Humans, Indicators and Reagents, Sample preparation, Ammonium acetate, Chromatography, High Pressure Liquid, Spectroscopy |
| الوصف: | Direct injection versus liquid-liquid extraction for post-dose human plasma sample analysis by high performance liquid chromatography with tandem mass spectrometry (LC/MS/MS) have been studied using a drug candidate compound. For the direct-injection method, an Oasis(R) HLB column (1 x 50 mm, 30 micrometer) was used as the on-line extraction column and a conventional Waters symmetry C18 column (3.9 x 50 mm, 5 micrometer) was used as the analytical column. Each plasma sample (100 microL) was mixed with 100 microL of a working solution of the internal standard in aqueous 0.05 M ammonium acetate (pH 6.9), and portions (10 microL) of these samples were then injected into the LC/MS/MS system. For the liquid-liquid extraction method, a YMC Basic C18 column (2.0 x 50 mm, 5 micrometer) was used as the analytical column. Each sample (0.5 mL) was extracted with methyl tert-butyl ether and the extract was reconstituted and injected into the LC/MS/MS system. The total analysis time for both methods was 2.0 min per sample. The accuracy, inter-day precision and intra-day precision obtained from the quality control samples were within 8% for both methods. The analysis results of post-dose human plasma samples showed that the deviations of 91% of the concentrations obtained using the direct-injection method were within +/-20% from the concentrations obtained using the liquid-liquid extraction method, and the overall average percentage deviation was -1.5%. The results showed that the two methods were equivalent in terms of total chromatographic run time, accuracy and precision. However, for a batch of 100 samples, the sample preparation time for the direct-injection method was only about 25% of the time required for liquid-liquid extraction. This decrease in sample preparation time resulted in the doubling of the overall sample analysis throughput. |
| تدمد: | 1097-0231 0951-4198 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::77dce33bf47020597fd7803692b97940 https://doi.org/10.1002/(sici)1097-0231(19991115)13:21<2125::aid-rcm763>3.0.co;2-h |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....77dce33bf47020597fd7803692b97940 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10970231 09514198 |
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