Alveolar epithelial type II cell apoptosis in vivo during resolution of keratinocyte growth factor-induced hyperplasia in the rat
| العنوان: | Alveolar epithelial type II cell apoptosis in vivo during resolution of keratinocyte growth factor-induced hyperplasia in the rat |
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| المؤلفون: | Robert J. Mason, Tianli Pan, Heinz Fehrenbach, Martin Müller, D Schuh, Roland Koslowski, Michael Kasper |
| المصدر: | Histochemistry and Cell Biology. 114:49-61 |
| بيانات النشر: | Springer Science and Business Media LLC, 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | Cell type, Fas Ligand Protein, Fibroblast Growth Factor 7, Time Factors, Histology, Immunoelectron microscopy, Cell, Apoptosis, Fas ligand, Bcl-2-associated X protein, Proto-Oncogene Proteins, In Situ Nick-End Labeling, medicine, Animals, fas Receptor, Fluorescent Antibody Technique, Indirect, Growth Substances, Microscopy, Immunoelectron, Molecular Biology, bcl-2-Associated X Protein, Hyperplasia, Membrane Glycoproteins, TUNEL assay, biology, Caspase 3, Epithelial Cells, Cell Biology, medicine.disease, Molecular biology, Rats, Inbred F344, Recombinant Proteins, Rats, Cell biology, Fibroblast Growth Factors, Pulmonary Alveoli, Medical Laboratory Technology, medicine.anatomical_structure, Proto-Oncogene Proteins c-bcl-2, Caspases, biology.protein, Fibroblast Growth Factor 10, Signal Transduction |
| الوصف: | Keratinocyte growth factor (KGF) induces rapid and transient hyperplasia of alveolar epithelial type II cells. We sought to determine components of the apoptotic process involved in the resolution of this hyperplasia and the fate of the apoptotic cells. Rats received intrabronchial instillation of 5 mg KGF/kg body weight or diluent. Lungs were fixed 1, 2, 3, 5, and 7 days later. Apoptosis was identified by TdT-mediated dUTP nick-end labeling (TUNEL), double-labeling for TUNEL and the type II cell marker MNF116, and electron microscopy. Fas, FasL, Bax, Bcl-2, and pro- and active caspase-3 were studied by immunohistochemistry. Changes were quantified by stereology. Cell type specificity was investigated by immunofluorescence double staining. Type II cells exhibited Fas, FasL, Bcl-2, and procaspase-3 irrespective of treatment and time. Immunoelectron microscopy revealed Fas at the apical type II cell membrane. Bax staining was prominent in controls (45-95% of type II cell surface fraction), markedly decreased during hyperplasia at days 2 (20-40%) and 3 (0-10%), and reappeared at day 7 (25-45%) when apoptosis was prominent. Remnants of apoptotic type II cells were incorporated in membrane-bound vacuoles of type II cell neighbors as well as alveolar macrophages. The results indicate that type II cells can enter the Fas/FasL/caspase-3 pathway regulated by Bax and Bcl-2. High Bcl-2:Bax levels favor type II cell survival and a low rate of apoptosis during hyperplasia. Low Bcl-2:Bax levels favor type II cell apoptosis during resolution. Because of time-dependent changes that occur within a short time, the KGF-treated rat lung provides a useful in vivo model to investigate apoptosis in the context of tissue remodeling and repair. |
| تدمد: | 1432-119X 0948-6143 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::782d66df9dff726c1f9885b20238aa12 https://doi.org/10.1007/s004180000157 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....782d66df9dff726c1f9885b20238aa12 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 1432119X 09486143 |
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