Evaluation of a fully automated von Willebrand factor assay panel for the diagnosis of von Willebrand disease
| العنوان: | Evaluation of a fully automated von Willebrand factor assay panel for the diagnosis of von Willebrand disease |
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| المؤلفون: | Luciano Baronciani, Paola Colpani, Marco Boscarino, Paolo Bucciarelli, Francesca Stufano, Flora Peyvandi, Maria Teresa Pagliari |
| المصدر: | Haemophilia. 26:298-305 |
| بيانات النشر: | Wiley, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Adult, congenital, hereditary, and neonatal diseases and abnormalities, 030204 cardiovascular system & hematology, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Von Willebrand factor, hemic and lymphatic diseases, von Willebrand Factor, medicine, Von Willebrand disease, Humans, Von Willebrand factor assay, Genetics (clinical), medicine.diagnostic_test, biology, business.industry, Hematology, General Medicine, Middle Aged, medicine.disease, von Willebrand Diseases, Fully automated, Immunoassay, Immunology, cardiovascular system, biology.protein, Female, Blood Coagulation Tests, business, circulatory and respiratory physiology, 030215 immunology |
| الوصف: | Introduction von Willebrand disease (VWD) diagnosis starts with first level tests: factor VIII coagulant activity, VWF antigen (VWF:Ag) and platelet-dependent VWF activity (VWF:RCo, VWF:Ab, VWF:GPIbR or VWF:GPIbM). The VWF collagen binding (VWF:CB) assay measures the binding capacity of von Willebrand factor (VWF) to collagen. Aim To assess, in previously diagnosed VWD patients, the performance of a fully automated chemiluminescent test panel including VWF:Ag, VWF:GPIbR and VWF:CB assays. Methods The patients, historically evaluated using in-house VWF:Ag and VWF:CB assays and an automated latex enhanced immunoassay VWF:GPIbR method, were re-evaluated using the VWF test panel HemosIL AcuStar. Results The VWF:GPIbR/VWF:Ag and VWF:CB/VWF:Ag obtained by means of AcuStar showed an overall good concordance with the corresponding data obtained at the time of the historical diagnosis. When discrepancies occurred, these were generally due to the lower VWF:CB/VWF:Ag obtained with AcuStar as compared with that obtained with the historical methods and this affected particularly the diagnosis of VWD type 2M. Together, the AcuStar VWF:GPIbR/VWF:Ag and VWF:CB/VWF:Ag were able to distinguish type 1 from types 2A, 2B and 2M, whereas no distinction was possible between type 2A and 2B. Conclusion The AcuStar panel offers a good performance in the differential diagnosis between VWD type 1 and 2A/2B patients. A high rate of coincidence with historical diagnosis was obtained for VWD types 3, 2A/2B and 1. Even though in some cases more tests (eg, RIPA/multimeric analysis) are needed to complete an accurate VWD classification, the AcuStar panel is considered a sensitive, rapid and reliable tool to diagnose VWD patients. |
| تدمد: | 1365-2516 1351-8216 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7833e58fe4a9fa3a7d9b87b9868b510b https://doi.org/10.1111/hae.13929 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....7833e58fe4a9fa3a7d9b87b9868b510b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13652516 13518216 |
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