Peripheral Administration of Tumor Necrosis Factor-Alpha Induces Neuroinflammation and Sickness but Not Depressive-Like Behavior in Mice
| العنوان: | Peripheral Administration of Tumor Necrosis Factor-Alpha Induces Neuroinflammation and Sickness but Not Depressive-Like Behavior in Mice |
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| المؤلفون: | Niels Hellings, Xavier Langlois, Jan A. Bouwknecht, Paul D. Acton, Steven Biesmans, Theo Meert, Nima Davoodi, Patrick De Haes, Luc Ver Donck, Rony Nuydens |
| المصدر: | BioMed Research International BioMed Research International, Vol 2015 (2015) |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Male, medicine.medical_specialty, Article Subject, Central nervous system, Interleukin-1beta, lcsh:Medicine, Mice, Transgenic, Biology, General Biochemistry, Genetics and Molecular Biology, Proinflammatory cytokine, Mice, Internal medicine, medicine, Animals, Neuroinflammation, Chemokine CCL2, Inflammation, General Immunology and Microbiology, Behavior, Animal, Depression, Interleukin-6, Tumor Necrosis Factor-alpha, Dentate gyrus, lcsh:R, Calcium-Binding Proteins, Microfilament Proteins, Brain, General Medicine, Interleukin-10, medicine.anatomical_structure, Endocrinology, Immunology, Systemic administration, Encephalitis, Tumor necrosis factor alpha, Cell activation, Biomarkers, Astrocyte, Research Article |
| الوصف: | Clinical observations indicate that activation of the TNF-αsystem may contribute to the development of inflammation-associated depression. Here, we tested the hypothesis that systemic upregulation of TNF-αinduces neuroinflammation and behavioral changes relevant to depression. We report that a single intraperitoneal injection of TNF-αin mice increased serum and brain levels of the proinflammatory mediators TNF-α, IL-6, and MCP-1, in a dose- and time-dependent manner, but not IL-1β. Protein levels of the anti-inflammatory cytokine IL-10 increased in serum but not in the brain. The transient release of immune molecules was followed by glial cell activation as indicated by increased astrocyte activation in bioluminescent Gfap-luc mice and elevated immunoreactivity against the microglial marker Iba1 in the dentate gyrus of TNF-α-challenged mice. Additionally, TNF-α-injected mice were evaluated in a panel of behavioral tests commonly used to study sickness and depressive-like behavior in rodents. Our behavioral data imply that systemic administration of TNF-αinduces a strong sickness response characterized by reduced locomotor activity, decreased fluid intake, and body weight loss. Depressive-like behavior could not be separated from sickness at any of the time points studied. Together, these results demonstrate that peripheral TNF-αaffects the central nervous system at a neuroimmune and behavioral level. |
| وصف الملف: | text/xhtml |
| تدمد: | 2314-6141 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::78b93fd05a82e5c622f340f0b3e59c33 https://pubmed.ncbi.nlm.nih.gov/26290874 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....78b93fd05a82e5c622f340f0b3e59c33 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23146141 |
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