Traction Force Measurements of Human Aortic Smooth Muscle Cells Reveal a Motor-Clutch Behavior
| العنوان: | Traction Force Measurements of Human Aortic Smooth Muscle Cells Reveal a Motor-Clutch Behavior |
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| المؤلفون: | Stéphane Avril, Alain Guignandon, Claudie Petit |
| المساهمون: | Avril, Stéphane, INSERM U1059, SAINBIOSE - Santé, Ingénierie, Biologie, Saint-Etienne (SAINBIOSE-ENSMSE), Centre Ingénierie et Santé (CIS-ENSMSE), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Ingénierie et Santé (CIS-ENSMSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT) |
| المصدر: | Molecular and Cellular Biomechanics Molecular and Cellular Biomechanics, 2019, ⟨10.32604/mcb.2019.06415⟩ Molecular & Cellular Biomechanics Molecular and Cellular Biomechanics, Tech Science Press, 2019, ⟨10.32604/mcb.2019.06415⟩ |
| بيانات النشر: | HAL CCSD, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Materials science, Cell biomechanics, medicine.medical_treatment, 0206 medical engineering, Biophysics, 02 engineering and technology, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Traction force microscopy, 03 medical and health sciences, 0302 clinical medicine, isolated cells, medicine, [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Mechanotransduction, Cell adhesion, Molecular Biology, Elastic modulus, mechanotransduction, Tractive force, Linear elasticity, fluorescent microscopy (FM), Stiffness, Cell Biology, General Medicine, smooth muscle cells (SMC), mechanosensitivity, Traction (orthopedics), musculoskeletal system, 020601 biomedical engineering, cardiovascular system, Molecular Medicine, medicine.symptom, traction force microscopy (TFM), aortic aneurysm, 030217 neurology & neurosurgery |
| الوصف: | International audience; The contractile behavior of smooth muscle cells (SMCs) in the aorta is an important determinant of growth, remodeling, and homeostasis. However, quantitative values of SMC basal tone have never been characterized precisely on individual SMCs. Therefore, to address this lack, we developed an in vitro technique based on Traction Force Microscopy (TFM). Aortic SMCs from a human lineage at low passages (4-7) were cultured 2 days in conditions promoting the development of their contractile apparatus and seeded on hydrogels of varying elastic modulus (1, 4, 12 and 25 kPa) with embedded fluorescent microspheres. After complete adhesion, SMCs were artificially detached from the gel by trypsin treatment. The microbeads movement was tracked and the deformation fields were processed with a mechanical model, assuming linear elasticity, isotropic material, plane strain, to extract the traction forces formerly applied by individual SMCs on the gel. Two major interesting and original observations about SMC traction forces were deduced from the obtained results: 1. they are variable but driven by cell dynamics and show an exponential distribution, with 40% to 80% of traction forces in the range 0-10 µN. 2. They depend on the substrate stiffness: the fraction of adhesion forces below 10 µN tend to decrease when the substrate stiffness increases, whereas the fraction of higher adhesion forces increases. As these two aspects of cell adhesion (variability and stiffness dependence) and the distribution of their traction forces can be predicted by the probabilistic motor-clutch model, we conclude that this model could be applied to SMCs. Further studies will consider stimulated contractility and primary culture of cells extracted from aneurysmal human aortic tissue. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1556-5297 1556-5300 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::78f1211239706e4027cb5095d1a0180b https://hal.science/hal-02405709 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....78f1211239706e4027cb5095d1a0180b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15565297 15565300 |
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