FBS/BSA media concentration determines CCCP’s ability to depolarize mitochondria and activate PINK1-PRKN mitophagy
| العنوان: | FBS/BSA media concentration determines CCCP’s ability to depolarize mitochondria and activate PINK1-PRKN mitophagy |
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| المؤلفون: | Christin Luft, Marc P.M. Soutar, Robin Ketteler, Marthe H.R. Ludtmann, Emily Annuario, Helene Plun-Favreau, Liam Kempthorne, Selina Wray |
| المصدر: | Autophagy |
| بيانات النشر: | Informa UK Limited, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Serum, 0301 basic medicine, Carbonyl Cyanide m-Chlorophenyl Hydrazone, Ubiquitin-Protein Ligases, PINK1, Mitochondrion, Electron Transport Complex III, 03 medical and health sciences, Mitophagy, Humans, Letter to the Editor, Molecular Biology, Membrane Potential, Mitochondrial, 030102 biochemistry & molecular biology, biology, Kinase, Healthy population, Autophagy, Serum Albumin, Bovine, Depolarization, Cell Biology, Mitochondrial Proton-Translocating ATPases, Culture Media, Mitochondria, Cell biology, Ubiquitin ligase, 030104 developmental biology, biology.protein, Lysosomes, Protein Kinases, HeLa Cells |
| الوصف: | Mitochondrial quality control is essential for maintaining a healthy population of mitochondria. Two proteins associated with Parkinson disease, the kinase PINK1 and the E3 ubiquitin ligase PRKN, play a central role in the selective degradation of heavily damaged mitochondria (mitophagy), thus avoiding their toxic accumulation. Most of the knowledge on PINK1-PRKN mitophagy comes from in vitro experiments involving the treatment of mammalian cells with high concentrations of mitochondrial uncouplers, such as CCCP. These chemicals have been shown to mediate off target effects, other than mitochondrial depolarization. A matter of controversy between mitochondrial physiologists and cell biologists is the discrepancy between concentrations of CCCP needed to activate mitophagy (usually >10 μM), when compared to the much lower concentrations used to depolarize mitochondria ( |
| وصف الملف: | application/pdf |
| تدمد: | 1554-8635 1554-8627 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::78fc96e5f36512a1ea3908ce6432e531 https://doi.org/10.1080/15548627.2019.1603549 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....78fc96e5f36512a1ea3908ce6432e531 |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 15548635 15548627 |
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