Phase I Study of MetXia-P450 Gene Therapy and Oral Cyclophosphamide for Patients with Advanced Breast Cancer or Melanoma
| العنوان: | Phase I Study of MetXia-P450 Gene Therapy and Oral Cyclophosphamide for Patients with Advanced Breast Cancer or Melanoma |
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| المؤلفون: | Adrian L. Harris, O. Kan, Richard Harrop, Martin Forster, Jan Steiner, Gael Deplanque, Miles W. Carroll, Andreas Makris, S. Kingsman, Linda White, D Talbot, J P Braybrooke, Stuart Naylor, Srinivasan Madhusudan, Andrew Slade, Rachel J. Gibson |
| المصدر: | Clinical Cancer Research. 11:1512-1520 |
| بيانات النشر: | American Association for Cancer Research (AACR), 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | Adult, Male, Cancer Research, Pathology, medicine.medical_specialty, Time Factors, Cyclophosphamide, Genetic Vectors, Administration, Oral, Breast Neoplasms, Enzyme-Linked Immunosorbent Assay, chemistry.chemical_compound, Breast cancer, Carcinoembryonic antigen, Cell Line, Tumor, Biopsy, medicine, Humans, Antineoplastic Agents, Alkylating, Melanoma, Aged, biology, medicine.diagnostic_test, business.industry, Mucin-1, Cancer, Oxidoreductases, N-Demethylating, Genetic Therapy, Middle Aged, medicine.disease, Immunohistochemistry, Nitrogen mustard, Carcinoembryonic Antigen, Cytochrome P-450 CYP2B6, Treatment Outcome, Oncology, chemistry, biology.protein, Cancer research, Female, Aryl Hydrocarbon Hydroxylases, business, Progressive disease, medicine.drug |
| الوصف: | Purpose: MetXia-P450 is a novel recombinant retroviral vector that encodes the human cytochrome P450 type 2B6 gene (CYP2B6), Escherichia coli lacZ, and neomycin resistance marker genes. Cytochrome P450 enzymes are primarily expressed in the liver and convert the prodrug cyclophosphamide to an active phosphoramide mustard and acrolein. Gene-based delivery of CYP2B6 to the tumor site leads to local prodrug activation and higher concentrations of the active metabolites at the target site. Experimental Design: MetXia-P450 was directly injected into metastatic cutaneous tumor nodules on days 1 and 2 and nodules biopsied on day 7. Oral cyclophosphamide (100 mg/m2) was administered between days 8 and 22. Subsequent cycles of oral cyclophosphamide were repeated for 2 of 4 weeks. Gene transfer levels in biopsy samples were measured by histologic and quantitative PCR analyses. Safety assessments were made using PCR for vector dissemination to the blood after injection and using PCR and serologic analyses to detect replicating virus. Secondary end points included clinical response, toxicity, and evaluation of antitumor immune responses by measurement of carcinoembryonic antigen and 5T4 antibodies. Results: Twelve patients with breast cancer (n = 9) and melanoma (n = 3) received three dose levels of MetXia-P450 (∼8 × 105, ∼8 × 106, and ∼8 × 107 lacZ transferring units/mL). The product was safe and well tolerated. The lacZ transgene was detected in biopsy material by immunohistochemistry in 10 of 12 patients and integrated viral sequences by PCR in 3 of 6 patients. One (8%) patient with breast cancer had a partial response and received 7 months of oral cyclophosphamide. Four (33%) patients had stable disease for ≥3 months and the rest had progressive disease. Preliminary immunologic analyses were suggestive of an antitumor response in two patients (partial response in one patient and stable disease in one patient). Conclusion: MetXia was safe and well tolerated. Gene transfer was detected at all dose levels, and the initial suggestion of an antitumor response indicates that MetXia-P450 should undergo further clinical assessment. |
| تدمد: | 1557-3265 1078-0432 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::791c9f4127519ae1a8e902b6a3f69f5b https://doi.org/10.1158/1078-0432.ccr-04-0155 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....791c9f4127519ae1a8e902b6a3f69f5b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15573265 10780432 |
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