ESR investigations on radical formation after ultraviolet irradiation of trypsin in various polar and nonpolar media
| العنوان: | ESR investigations on radical formation after ultraviolet irradiation of trypsin in various polar and nonpolar media |
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| المؤلفون: | Nemec Hw, Hans Mönig |
| المصدر: | Radiation and Environmental Biophysics. 11:227-238 |
| بيانات النشر: | Springer Science and Business Media LLC, 1974. |
| سنة النشر: | 1974 |
| مصطلحات موضوعية: | Free Radicals, Ultraviolet Rays, Radical, Biophysics, Photochemistry, law.invention, Cyclohexanes, law, Alkanes, Nitriles, Ultraviolet light, medicine, Trypsin, Irradiation, Electron paramagnetic resonance, Spectroscopy, Bond cleavage, General Environmental Science, Radiochemistry, Radiation, Chemistry, Electron Spin Resonance Spectroscopy, Unpaired electron, Alcohols, Chloroform, Ethers, medicine.drug |
| الوصف: | Crystalline trypsin was irradiated with ultraviolet light of 254 nm in various oxygen-free suspension media at a temperature of 278 K. Using electron spin resonance spectroscopy long-living free radicals were detected in the enzyme. Unpaired electrons are mainly localized at Cα-atoms of the polypeptide chain and at the sulphur of damaged disulfide bridges. The ultraviolet irradiation of trypsin leads to greater yields of thiyl radicals RS · in nonpolar media than in polar ones. If the dielectric constant is chosen as a measure of polarity a relationship between the relative concentration of thiyl radicals [RS ·]rel and the dielectric constante in form of [RS ·]rel ∼ 1/e is obtained. The manner how the suspension media may influence the formation of thiyl radicals is discussed. The results allow the conclusion that the radiation-induced scission of disulflde bonds is of significance for the inactivation of trypsin. |
| تدمد: | 1432-2099 0301-634X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::79bfe3fc54307ab53d1744e17ad74d79 https://doi.org/10.1007/bf01323192 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....79bfe3fc54307ab53d1744e17ad74d79 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14322099 0301634X |
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