Comprehensive evaluation of differential long non-coding RNA and gene expression in patients with cartilaginous endplate degeneration of cervical vertebra
| العنوان: | Comprehensive evaluation of differential long non-coding RNA and gene expression in patients with cartilaginous endplate degeneration of cervical vertebra |
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| المؤلفون: | Rui Ding, Xijuan Liu, Tianlong Wu, Chongzhi Pang, Jian Zhang, Jingyu Jia, Jinghong Yuan, Xigao Cheng, Shen Hu |
| المصدر: | Experimental and Therapeutic Medicine |
| بيانات النشر: | D.A. Spandidos, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Regulation of gene expression, Cancer Research, Messenger RNA, Competing endogenous RNA, mRNAs, General Medicine, Computational biology, Articles, bioinformatics, Biology, Long non-coding RNA, 03 medical and health sciences, long non-coding RNAs, 030104 developmental biology, 0302 clinical medicine, Immunology and Microbiology (miscellaneous), 030220 oncology & carcinogenesis, Gene expression, microRNA, competitive endogenous RNA, endplate cartilage, KEGG, Gene |
| الوصف: | Long non-coding RNAs (lncRNAs) are emerging as key regulators in gene expression; however, little is currently known regarding their role in cartilaginous endplate (CE) degeneration (CED) of cervical vertebra. The present study aimed to investigate the expression levels of lncRNAs and analyze their potential functions in CED of cervical vertebra in patients with cervical fracture and cervical spondylosis. Human competitive endogenous RNA (ceRNA) array was used to analyze lncRNA and mRNA expression levels in CE samples from patients with cervical fracture and cervical spondylosis, who received anterior cervical discectomy and fusion. Differentially expressed lncRNAs (DELs) or differentially expressed genes (DEGs) were identified and functionally analyzed, using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. An lncRNA-microRNA(miRNA)-mRNA ceRNA regulatory network was constructed based on the DELs and DEGs, and the ceRNA network was visualized using Cytoscape 3.7.2 software. In total, one downregulated mRNA, one upregulated miRNA and five downstream regulated lncRNAs were identified using reverse transcription-quantitative PCR in CED and healthy CE samples. A total of 369 lncRNAs and 246 mRNAs were identified as differentially expressed in CE. The GO and KEGG analyses demonstrated that the majority of GO and KEGG enrichments were associated with CED. Furthermore, a ceRNA network was established, including 168 putative miRNA response elements, 189 upregulated and 37 downregulated lncRNAs and 47 upregulated and 10dow regulated DEGs. The present study analyzed the function of DEGs in the ceRNA network and filtered out the same items as in DEG-function enrichment analysis. These results provide a new perspective for an improved understanding of ceRNA-mediated gene regulation in cervical spondylosis, and provide a novel theoretical basis for further studies on the function of lncRNA in cervical spondylosis. However, further experiments are required to validate the results of the present study. |
| اللغة: | English |
| تدمد: | 1792-1015 1792-0981 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::79e31ee98a91c4138e7a8f0bdab56830 http://europepmc.org/articles/PMC7664616 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....79e31ee98a91c4138e7a8f0bdab56830 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17921015 17920981 |
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