Comparative transcriptome and metabolite profiling of four tissues from Alisma orientale (Sam.) Juzep reveals its inflorescence developmental and medicinal characteristics
| العنوان: | Comparative transcriptome and metabolite profiling of four tissues from Alisma orientale (Sam.) Juzep reveals its inflorescence developmental and medicinal characteristics |
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| المؤلفون: | Lu Xuehua, Rongqing Xu, Yamin Zhang, Li Lisha, Wenjin Lin, Xu Xiaomei, Feng-Ling Sun |
| المصدر: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019) |
| بيانات النشر: | Nature Publishing Group UK, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Plant molecular biology, ved/biology.organism_classification_rank.species, Farnesyl pyrophosphate, lcsh:Medicine, UniGene, Biology, Plant Roots, Article, Transcriptome, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Metabolomics, Gene Expression Regulation, Plant, Alisma orientale, Inflorescence, lcsh:Science, Transcriptomics, Cholestenones, Multidisciplinary, Plants, Medicinal, ved/biology, cDNA library, Gene Expression Profiling, lcsh:R, nutritional and metabolic diseases, Reproducibility of Results, Molecular Sequence Annotation, Triterpenes, Gene expression profiling, Plant Leaves, 030104 developmental biology, Gene Ontology, chemistry, Biochemistry, Alisma, Linear Models, lcsh:Q, Secondary metabolism, 030217 neurology & neurosurgery, Microsatellite Repeats |
| الوصف: | Alisma orientale (Sam.) Juzep (A. orientale) is an important medicinal plant in traditional Chinese medicine. In this study, de novo RNA-seq of A. orientale was performed based on the cDNA libraries from four different tissues, roots, leaves, scapes and inflorescences. A total of 41,685 unigenes were assembled, 25,024 unigene functional annotations were obtained by searching against the five public sequence databases, and 3,411 simple sequence repeats in A. orientale were reported for the first time. 15,402 differentially expressed genes were analysed. The morphological characteristics showed that compared to the other tissues, the leaves had more chlorophyll, the scapes had more vascular bundles, and the inflorescences contained more starch granules and protein. In addition, the metabolic profiles of eight kinds of alisols metabolite profiling, which were measured by ultra-Performance liquid chromatography-triple quadrupole-mass spectrometry showed that alisol B 23-acetate and alisol B were the major components of the four tissues at amounts of 0.068~0.350 mg/g and 0.046~0.587 mg/g, respectively. In addition, qRT-PCR validated that farnesyl pyrophosphate synthase and 3-hydroxy-3-methylglutaryl-CoA reductase should be considered the critical candidate genes involved in alisol biosynthesis. These transcriptome and metabolic profiles of A. orientale may help clarify the molecular mechanisms underlying the medicinal characteristics of A. orientale. |
| اللغة: | English |
| تدمد: | 2045-2322 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7a45ba64e5c977ae11f60370df99f570 http://europepmc.org/articles/PMC6707231 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....7a45ba64e5c977ae11f60370df99f570 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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