Systematical Engineering of Synthetic Yeast for Enhanced Production of Lycopene
| العنوان: | Systematical Engineering of Synthetic Yeast for Enhanced Production of Lycopene |
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| المؤلفون: | Wang Yun, Yue Shen, Jin-Tao Zhang, Ao Chen, Wen-Wei Zhang, Shu-Wen Wang, Shu-Jie Wang, Long-Ying Liu, Tai Chen, Wang Yong, Tsan-Yu Chiu, Yu Zhang, Zhi Ping |
| المصدر: | Bioengineering Volume 8 Issue 1 Bioengineering, Vol 8, Iss 14, p 14 (2021) |
| بيانات النشر: | MDPI, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0106 biological sciences, lycopene production optimization, Heterologous, Bioengineering, SCRaMbLE, lcsh:Technology, 01 natural sciences, Article, 03 medical and health sciences, Synthetic biology, chemistry.chemical_compound, 010608 biotechnology, Biomanufacturing, lcsh:QH301-705.5, 030304 developmental biology, 0303 health sciences, combinatorial assembly, Strain (chemistry), lcsh:T, Host (biology), Chemistry, Yeast, Lycopene, synthetic yeast, lcsh:Biology (General), Biochemistry, Yield (chemistry) |
| الوصف: | Synthetic biology allows the re-engineering of biological systems and promotes the development of bioengineering to a whole new level, showing great potential in biomanufacturing. Here, in order to make the heterologous lycopene biosynthesis pathway compatible with the host strain YSy 200, we evolved YSy200 using a unique Synthetic Chromosome Rearrangement and Modification by LoxP-mediated Evolution (SCRaMbLE) system that is built in the Sc2.0 synthetic yeast. By inducing SCRaMbLE, we successfully identified a host strain YSy201 that can be served as a suitable host to maintain the heterologous lycopene biosynthesis pathway. Then, we optimized the lycopene biosynthesis pathway and further integrated into the rDNA arrays of YSy201 to increase its copy number. In combination with culturing condition optimization, we successfully screened out the final yeast strain YSy222, which showed a 129.5-fold increase of lycopene yield in comparison with its parental strain. Our work shows that, the strategy of combining the engineering efforts on both the lycopene biosynthesis pathway and the host strain can improve the compatibility between the heterologous pathway and the host strain, which can further effectively increase the yield of the target product. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2306-5354 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7a774124fedb8cf93f452fe91c3e6b25 http://europepmc.org/articles/PMC7833358 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....7a774124fedb8cf93f452fe91c3e6b25 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23065354 |
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