Chromosomal diversification of diploid number, heterochromatin and rDNAs in two species of Phanaeus beetles (Scarabaeidae, Scarabaeinae)
| العنوان: | Chromosomal diversification of diploid number, heterochromatin and rDNAs in two species of Phanaeus beetles (Scarabaeidae, Scarabaeinae) |
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| المؤلفون: | Cesar Martins, Amanda Paulino De Arcanjo, Rita de Cássia de Moura, Diogo Cavalcanti Cabral-de-Mello, Maria José de Souza |
| المساهمون: | Universidade Federal de Pernambuco (UFPE), Universidade de Pernambuco, Universidade Estadual Paulista (Unesp) |
| المصدر: | Genetics and Molecular Biology v.36 n.3 2013 Genetics and Molecular Biology Sociedade Brasileira de Genética (SBG) instacron:SBG Genetics and Molecular Biology, Volume: 36, Issue: 3, Pages: 341-346, Published: 16 AUG 2013 Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Genetics and Molecular Biology, Vol 36, Iss 3, Pp 341-346 (2013) |
| بيانات النشر: | FapUNIFESP (SciELO), 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Subfamily, lcsh:QH426-470, Heterochromatin, ribosome DNA, DNA sequence, chromosome evolution, Phanaeus, DNA 18S, animal tissue, male, FISH, Genus, Genetics, Constitutive heterochromatin, base pairing, DNA 5S, Scarabaeinae, fluorescence in situ hybridization, Molecular Biology, Ribosomal DNA, diploidy, pericentric chromosome inversion, chromosomal diversification, nonhuman, biology, Phanaeus (Notiophanaeus) splendidulus, beetle, fluorochrome staining, repetitive DNA, heterochromatin, nucleotide sequence, Phanaeus (Notiophanaeus) chalcomelas, Karyotype, gene mapping, autosome, biology.organism_classification, C banding, chromosome NOR, karyotype, lcsh:Genetics, chromosomes and chromosomal phenomena, Ploidy, Animal Genetics, Research Article |
| الوصف: | Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:30:40Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:37:59Z : No. of bitstreams: 1 2-s2.0-84883275469.pdf: 959576 bytes, checksum: eaf44ccb1bbddf766c0f082889d0d22d (MD5) Made available in DSpace on 2014-05-27T11:30:40Z (GMT). No. of bitstreams: 0 Previous issue date: 2013-09-05 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundacao de Amparo a Ciência e Tecnologia do Estado de Pernambuco Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) The genus Phanaeus is included in the tribe Phanaeini, one of the most diverse tribes within the subfamily Scarabaeinae in terms of chromosomal characteristics. However, so far the species of this genus were not studied with differential cytogenetic techniques, limiting any inference of the probable mechanisms responsible for this diversity. In this work, several techniques were applied with the aim of cytogenetically characterizing two Phanaeus species. The karyotype found for Phanaeus (Notiophanaeus) chalcomelas was 2n = 12, neo-XY, and that of P. (N.) splendidulus was 2n = 20, Xyp, considered primitive for the family Scarabaeidae. The chromosomes of both species showed a high amount of constitutive heterochromatin (CH), with blocks rich in base pairs GC (CMA3 +). Moreover, in P. (N.) chalcomelas the marks revealed by C-banding and fluorochrome staining were different in size, showing CH variability. Sites of 18S ribosomal DNA (rDNA) were identified in one autosomal pair of P. (N.) chalcomelas and in five autosomal pairs of P. (N.) splendidulus. On the other hand, only one autosomal pair exhibited 5S rDNA sequences in these species. The results suggest that the karyotype differentiation of the Phanaeus species studied here involved pericentric inversions and centric fusions, as well as mechanisms related to amplification and dispersion of CH and rDNA sequences. © 2013, Sociedade Brasileira de Genética. Departamento de Genética Centro de Ciências Biológicas Universidade Federal de Pernambuco, Recife, PE Departamento de Biologia Instituto de Ciências Biológicas Universidade de Pernambuco, Recife, PE Departamento de Biologia Instituto de Biociências Universidade Estadual Paulista 'Júlio de Mesquita Filho', Rio Claro, SP Departamento de Morfologia Instituto de Biociências Universidade Estadual Paulista 'Júlio de Mesquita Filho', Botucatu, SP Departamento de Biologia Instituto de Biociências Universidade Estadual Paulista 'Júlio de Mesquita Filho', Rio Claro, SP Departamento de Morfologia Instituto de Biociências Universidade Estadual Paulista 'Júlio de Mesquita Filho', Botucatu, SP FACEPE - BPD-003.2.02/08 e APQ-0464-2.02/10 |
| وصف الملف: | text/html |
| تدمد: | 1678-4685 1415-4757 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7a8c69fb8dbe74a5a364b22fe8b79e62 https://doi.org/10.1590/s1415-47572013005000031 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....7a8c69fb8dbe74a5a364b22fe8b79e62 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16784685 14154757 |
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