Expression and significance of MMP2 and HIF-1α in hepatocellular carcinoma
| العنوان: | Expression and significance of MMP2 and HIF-1α in hepatocellular carcinoma |
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| المؤلفون: | Weixing Wang, You‑Ming Ding, Bing Wang, Bin Wang, Ping Fan, Jun‑Hui Xu |
| المصدر: | Oncology Letters |
| بيانات النشر: | D.A. Spandidos, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Cancer Research, Pathology, medicine.medical_specialty, MMP2, Oncogene, Articles, hepatocellular carcinoma, Biology, medicine.disease, Molecular medicine, digestive system diseases, Metastasis, Real-time polymerase chain reaction, Oncology, matrix metalloproteinase 2, Hepatocellular carcinoma, Gene expression, medicine, gene expression, Immunohistochemistry, hypoxia-inducible factor 1α |
| الوصف: | Hepatocellular carcinoma (HCC) is a serious threat to human health. HCC is a malignant tumor and its invasion and metastasis are the result of multigene interactions. Matrix metalloproteinase-2 (MMP-2) is capable of degrading the majority of components of the extracellular matrix and is regarded to closely correlate with tumor invasion and metastasis. Furthermore, the hypoxia-inducible factor 1α (HIF-1α) is an important transcription factor, which is closely associated with the process of tumor growth. The aim of the present study was to investigate the expression of MMP2 and HIF-1α) in HCC, and the relationship between MMP2/HIF-1α protein expression and the clinical/pathological characteristics of HCC. The mRNA levels of MMP2 and HIF-1α were detected in 32 cases of HCC and the corresponding normal adjacent tissues with fluorescence-based quantitative polymerase chain reaction (qPCR). The protein expression of MMP2 and HIF-1α was assessed in 45 HCC cases and 33 cases of corresponding normal adjacent tissue, using immunohistochemical methods. The association between MMP2/HIF-1α and pathological features of HCC, and the correlation between MMP2 and HIF-1α were analyzed. The Kaplan-Meier method was used to assess the impact of MMP2 and HIF-1α expression on survival. The fluorescence-based qPCR demonstrated that MMP2 and HIF-1α mRNA expression levels in the HCC tissues were 0.84±0.17 and 0.87±0.11, respectively, which were significantly higher than those in the adjacent normal tissues (0.70±0.13 and 0.68±0.13, respectively; P0.05). However, there was significant correlation between MMP2 or HIF-1α protein expression and tumor size, metastasis, presence of a capsule and clinical TNM staging of HCC. Their expression also had a significant effect on patient survival time. In conclusion, MMP2 and HIF-1α are overexpressed in HCC, and the MMP2 signaling pathway may promote the development of HCC together with HIF-1α. |
| اللغة: | English |
| تدمد: | 1792-1082 1792-1074 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7a9741bc4810f0d25ab51b3c28e592c4 http://europepmc.org/articles/PMC4081179 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....7a9741bc4810f0d25ab51b3c28e592c4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17921082 17921074 |
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