Neutrophils, neutrophil extracellular traps and interleukin-17 associate with the organisation of thrombi in acute myocardial infarction
| العنوان: | Neutrophils, neutrophil extracellular traps and interleukin-17 associate with the organisation of thrombi in acute myocardial infarction |
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| المؤلفون: | Xiaofei Li, Claire Mackaay, Peter Teeling, Onno J. de Boer, Hanneke J. Ploegmakers, Chris M. van der Loos, Mat J.A.P. Daemen, Allard C. van der Wal, Robbert J. de Winter |
| المساهمون: | ACS - Amsterdam Cardiovascular Sciences, Pathology, Cardiology |
| المصدر: | Thrombosis and haemostasis, 109(2), 290-297. Schattauer GmbH |
| بيانات النشر: | Georg Thieme Verlag KG, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | 0301 basic medicine, Pathology, medicine.medical_specialty, Neutrophils, Blotting, Western, Myocardial Infarction, Neutrophil Activation, Histones, 03 medical and health sciences, 0302 clinical medicine, Western blot, Rosaniline Dyes, Humans, Medicine, RNA, Messenger, Thrombus, Peroxidase, Thrombectomy, biology, medicine.diagnostic_test, Reverse Transcriptase Polymerase Chain Reaction, business.industry, Interleukin-17, Interleukin, Thrombosis, Hematology, Neutrophil extracellular traps, medicine.disease, Immunohistochemistry, Staining, Blot, 030104 developmental biology, 030220 oncology & carcinogenesis, Myeloperoxidase, Neutrophil elastase, biology.protein, Leukocyte Elastase, business, Biomarkers |
| الوصف: | SummaryNeutrophils are important cellular sources of interleukin (IL) 17A and –F. Moreover, upon activation neutrophils are able to excrete chromatin embedded with components from their cytoplasmic granules to form ‘neutrophil extracellular traps’ (NETs). Recent studies suggested that NETs contribute to thrombosis by promoting fibrin deposition and platelet aggregation. IL17A may also promote thrombosis by enhancing platelet aggregation. In the present study we investigated the presence of neutrophils, NETs and IL17A and –F in coronary thrombosuction specimens obtained from patients after acute myocardial infarction. Neutrophils and NETs were identified using histochemical (H&E, Feulgen procedure) and immunohistochemical stainings (Histone H1, myeloperoxidase, neutrophil elastase) in 15 fresh, 15 lytic and 15 organised thrombi. The presence and distribution of IL17A and –F was studied using (immuno)histochemical double staining and spectral image analysis, rtPCR and Western blot. High numbers of neutrophils are present (10–30% of the thrombus mass) in fresh and lytic, but not in organized thrombus. NETs were frequently observed in fresh (4/15) and lytic (12/15), but never in organised thrombus specimens. Double staining combining the Feulgen reaction with Histone- H1, MPO or neutrophil elastase confirmed colocalisation with DNA. Cytoplasmatic IL17A/F staining was found in the majority of the neutrophils, extracellularly and in NETs. Western blotting confirmed the presence of IL17A and IL17F in thrombus specimens. In conclusion, a large burden of neutrophils, neutrophil extracellular traps and IL17A and –F are important constituents of fresh and lytic thrombus after acute myocardial infarction. The specific colocalisation of these indicates a role during thrombus stabilisation and growth. |
| تدمد: | 2567-689X 0340-6245 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7aa792d8b97c32d2e71bb224f7b4f477 https://doi.org/10.1160/th12-06-0425 |
| حقوق: | RESTRICTED |
| رقم الأكسشن: | edsair.doi.dedup.....7aa792d8b97c32d2e71bb224f7b4f477 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2567689X 03406245 |
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