Chronic inflammation is a feature of Achilles tendinopathy and rupture
| العنوان: | Chronic inflammation is a feature of Achilles tendinopathy and rupture |
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| المؤلفون: | Bridget Watkins, Graham Wells, Simon Wood, Fernando O. Martinez, Natasha Jones, Kim Wheway, Hamish Reid, Andrew Carr, Robert Hedley, J L Newton, Stephanie G. Dakin, Louise Appleton, Stephen Gwilym |
| المصدر: | British Journal of Sports Medicine |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Adult, Male, 0301 basic medicine, Pathology, medicine.medical_specialty, Stromal cell, tendon, Biopsy, medicine.medical_treatment, Hamstring Muscles, Physical Therapy, Sports Therapy and Rehabilitation, Inflammation, Achilles Tendon, immunology, Young Adult, 03 medical and health sciences, 0302 clinical medicine, medicine, Humans, Orthopedics and Sports Medicine, Rotator cuff, Cells, Cultured, Aged, Rupture, Achilles tendon, business.industry, 030229 sport sciences, General Medicine, Middle Aged, medicine.disease, Tendon, 030104 developmental biology, medicine.anatomical_structure, Cytokine, Tendinopathy, Original Article, Female, Stromal Cells, medicine.symptom, business, Biomarkers, Hamstring, orthopaedics |
| الوصف: | BackgroundRecent investigation of human tissue and cells from positional tendons such as the rotator cuff has clarified the importance of inflammation in the development and progression of tendon disease. These mechanisms remain poorly understood in disease of energy-storing tendons such as the Achilles. Using tissue biopsies from patients, we investigated if inflammation is a feature of Achilles tendinopathy and rupture.MethodsWe studied Achilles tendon biopsies from symptomatic patients with either mid-portion tendinopathy or rupture for evidence of abnormal inflammatory signatures. Tendon-derived stromal cells from healthy hamstring and diseased Achilles were cultured to determine the effects of cytokine treatment on expression of inflammatory markers.ResultsTendinopathic and ruptured Achilles highly expressed CD14+ and CD68+ cells and showed a complex inflammation signature, involving NF-κB, interferon and STAT-6 activation pathways. Interferon markers IRF1 and IRF5 were highly expressed in tendinopathic samples. Achilles ruptures showed increased PTGS2 and interleukin-8 expression. Tendinopathic and ruptured Achilles tissues expressed stromal fibroblast activation markers podoplanin and CD106. Tendon cells isolated from diseased Achilles showed increased expression of pro-inflammatory and stromal fibroblast activation markers after cytokine stimulation compared with healthy hamstring tendon cells.ConclusionsTissue and cells derived from tendinopathic and ruptured Achilles tendons show evidence of chronic (non-resolving) inflammation. The energy-storing Achilles shares common cellular and molecular inflammatory mechanisms with functionally distinct rotator cuff positional tendons. Differences seen in the profile of ruptured Achilles are likely to be attributable to a superimposed phase of acute inflammation and neo-vascularisation. Strategies that target chronic inflammation are of potential therapeutic benefit for patients with Achilles tendon disease. |
| تدمد: | 1473-0480 0306-3674 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7ab36cc36451cff0dd896cef065e6f58 http://ora.ox.ac.uk/objects/uuid: |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....7ab36cc36451cff0dd896cef065e6f58 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14730480 03063674 |
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