The replication timing program of the Chinese hamster β-globin locus is established coincident with its repositioning near peripheral heterochromatin in early G1 phase
| العنوان: | The replication timing program of the Chinese hamster β-globin locus is established coincident with its repositioning near peripheral heterochromatin in early G1 phase |
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| المؤلفون: | Feng Li, Susan M. Keezer, Jianhua Chen, David M. Gilbert, Mark C. Butler, Masako Izumi |
| المصدر: | The Journal of Cell Biology |
| بيانات النشر: | The Rockefeller University Press, 2001. |
| سنة النشر: | 2001 |
| مصطلحات موضوعية: | DNA Replication, Time Factors, Heterochromatin, Xenopus, Mitosis, Locus (genetics), CHO Cells, Article, S Phase, 03 medical and health sciences, 0302 clinical medicine, Cricetinae, Dihydrofolate reductase, medicine, β-globin, DNA replication, cell cycle, heterochromatin, nuclear organization, Animals, Research Articles, In Situ Hybridization, Fluorescence, 030304 developmental biology, Cell Nucleus, 0303 health sciences, Replication timing, biology, Chinese hamster ovary cell, G1 Phase, Cell Biology, DNA, Molecular biology, Globins, Cell nucleus, Tetrahydrofolate Dehydrogenase, medicine.anatomical_structure, Bromodeoxyuridine, biology.protein, 030217 neurology & neurosurgery |
| الوصف: | We have examined the dynamics of nuclear repositioning and the establishment of a replication timing program for the actively transcribed dihydrofolate reductase (DHFR) locus and the silent beta-globin gene locus in Chinese hamster ovary cells. The DHFR locus was internally localized and replicated early, whereas the beta-globin locus was localized adjacent to the nuclear periphery and replicated during the middle of S phase, coincident with replication of peripheral heterochromatin. Nuclei were prepared from cells synchronized at various times during early G1 phase and stimulated to enter S phase by introduction into Xenopus egg extracts, and the timing of DHFR and beta-globin replication was evaluated in vitro. With nuclei isolated 1 h after mitosis, neither locus was preferentially replicated before the other. However, with nuclei isolated 2 or 3 h after mitosis, there was a strong preference for replication of DHFR before beta-globin. Measurements of the distance of DHFR and beta-globin to the nuclear periphery revealed that the repositioning of the beta-globin locus adjacent to peripheral heterochromatin also took place between 1 and 2 h after mitosis. These results suggest that the CHO beta-globin locus acquires the replication timing program of peripheral heterochromatin upon association with the peripheral subnuclear compartment during early G1 phase. |
| اللغة: | English |
| تدمد: | 1540-8140 0021-9525 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7aca2c29be98bc05dea400131b3ed709 http://europepmc.org/articles/PMC1255917 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....7aca2c29be98bc05dea400131b3ed709 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15408140 00219525 |
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