Smad‑binding decoy reduces extracellular matrix expression in human hypertrophic scar fibroblasts
| العنوان: | Smad‑binding decoy reduces extracellular matrix expression in human hypertrophic scar fibroblasts |
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| المؤلفون: | Taavi Lehto, Yiqi Seow, Samir El Andaloussi, Kiat Whye Kong, Chen Fan |
| المصدر: | Molecular Medicine Reports |
| بيانات النشر: | Spandidos Publications, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | collagen, 0301 basic medicine, Cancer Research, Cicatrix, Hypertrophic, Primary Cell Culture, Cell, Smad Proteins, SMAD, Biochemistry, Collagen Type I, Transforming Growth Factor beta1, Extracellular matrix, 03 medical and health sciences, Hypertrophic scar, 0302 clinical medicine, Genetics, medicine, Extracellular, Humans, SMAD binding, skin and connective tissue diseases, Molecular Biology, transforming growth factor-β1, Cells, Cultured, Smad, Skin, Chemistry, Articles, hypertrophic scar, Fibroblasts, medicine.disease, Actins, Extracellular Matrix, Cell biology, 030104 developmental biology, medicine.anatomical_structure, Oncology, 030220 oncology & carcinogenesis, Molecular Medicine, Signal transduction, Decoy, Signal Transduction |
| الوصف: | The exact mechanisms underlying hypertrophic scarring is yet to be fully understood. However, excessive collagen deposition by fibroblasts has been demonstrated to result in hypertrophic scar formation, and collagen synthesis in dermal fibroblasts is regulated by the transforming growth factor‑β1/Smad signaling pathway. In view of this, a Smad‑binding decoy was designed and its effects on hypertrophic scar‑derived human skin fibroblasts was evaluated. The results of the present study revealed that the Smad decoy attenuates the total amount of collagen, collagen I and Smad2/3 expression in scar fibroblasts. Data from RNA sequencing indicated that the Smad decoy induced more than 4‑fold change in 178 genes, primarily associated with to the extracellular matrix, compared with the untreated control. In addition, results from quantitative real‑time polymerase chain reaction further confirmed that the Smad decoy significantly attenuated the expression of extracellular matrix‑related genes, including COL1A1, COL1A2 and COL3A1. Furthermore, the Smad decoy reduced transforming growth factor‑β1‑induced collagen deposition in scar fibroblasts. Data generated from the present study provide evidence supporting the use of the Smad decoy as a potential hypertrophic scar treatment. |
| تدمد: | 1791-3004 1791-2997 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b326babddd6a6806c64bfe5c9a53023 https://doi.org/10.3892/mmr.2020.11549 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....7b326babddd6a6806c64bfe5c9a53023 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17913004 17912997 |
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