التفاصيل البيبلوغرافية
| العنوان: |
Designing fluorescent biosensors using circular permutations of riboswitches |
| المؤلفون: |
Johnny Truong, Yu-Fang Hsieh, Guifang Jia, Lynda Truong, Ming C. Hammond |
| المصدر: |
Truong, J; Hsieh, YF; Truong, L; Jia, G; & Hammond, MC. (2018). Designing fluorescent biosensors using circular permutations of riboswitches. Methods, 143, 102-109. doi: 10.1016/j.ymeth.2018.02.014. UC Berkeley: Retrieved from: http://www.escholarship.org/uc/item/80g636h7 |
| بيانات النشر: |
Elsevier BV, 2018. |
| سنة النشر: |
2018 |
| مصطلحات موضوعية: |
0301 basic medicine, Riboswitch, S-Adenosylmethionine, Transcription, Genetic, 1.1 Normal biological development and functioning, Aptamer, Clinical Sciences, Bioengineering, Biosensing Techniques, macromolecular substances, Computational biology, Ligands, Aptamers, Fluorescence, Article, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, Genetic, Underpinning research, Molecular Biology, Extramural, Chemistry, technology, industry, and agriculture, RNA, Aptamers, Nucleotide, Circular permutation in proteins, Ligand (biochemistry), 030104 developmental biology, Nucleic Acid Conformation, Generic health relevance, Transcription, Nucleotide, Biosensor |
| الوصف: |
© 2018 Elsevier Inc. RNA-based fluorescent (RBF) biosensors have been applied to detect a variety of metabolites in vitro and in live cells. They are designed by combining the ligand sensing domain of natural riboswitches with in vitro selected fluorogenic aptamers. Different biosensor topologies have been developed to accommodate the diversity of riboswitch structures. Here we show that circular permutation of the riboswitch ligand sensing domain also gives functional biosensors, using the SAM-I riboswitch as our model. We reveal that this design can enhance fluorescence turn-on and ligand binding affinity compared to the non-permuted topology. |
| وصف الملف: |
application/pdf |
| تدمد: |
1046-2023 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b3958d9bcbcccfb523ceff852251c9e
https://doi.org/10.1016/j.ymeth.2018.02.014 |
| حقوق: |
OPEN |
| رقم الأكسشن: |
edsair.doi.dedup.....7b3958d9bcbcccfb523ceff852251c9e |
| قاعدة البيانات: |
OpenAIRE |
