Evaluation of quantitative PCR for early diagnosis of Pseudomonas aeruginosa infection in cystic fibrosis: a prospective cohort study
| العنوان: | Evaluation of quantitative PCR for early diagnosis of Pseudomonas aeruginosa infection in cystic fibrosis: a prospective cohort study |
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| المؤلفون: | I. Frachon, M.-R. Munck, A. Dirou, D. Horeau-Langlard, M.-P. Pelletier, Christopher Payan, I. Danner-Boucher, J. Caillon, G. Le Gal, Gilles Rault, J. Le Bihan, Sylvain Rosec, S. Ramel, Claude Férec, Elise Poulhazan, Emmanuel Nowak, Geneviève Héry-Arnaud, K. Revert, A. Haloun, S. Gouriou, R. Le Berre, V. David |
| المساهمون: | CIC Brest, Université de Brest (UBO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hôpital de la Cavale Blanche, Thérapeutiques cliniques et expérimentales des infections (EA 3826) (EA 3826), Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Neurosciences cognitives (NC), Université Sciences et Technologies - Bordeaux 1-Centre National de la Recherche Scientifique (CNRS), Département de Médecine Interne et Pneumologie [Brest] (DMIP - Brest), Centre Hospitalier Régional Universitaire de Brest (CHRU Brest), Génétique moléculaire et génétique épidémiologique, Université de Brest (UBO)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Ressources et de Compétences de la Mucoviscidose (CRCM), Centre de Perharidy-Cellule de Coordination du Réseau Mucoviscidose (CECOREM), Groupe d'Etude de la Thrombose de Bretagne Occidentale (GETBO), Université de Brest (UBO)-Institut Brestois Santé Agro Matière (IBSAM), Université de Brest (UBO)-Université de Brest (UBO), Biologie et physiologie des états septiques, IFR114-Université de Lille, Droit et Santé, Calvez, Ghislaine |
| المصدر: | Clinical Microbiology and Infection Clinical Microbiology and Infection, Elsevier for the European Society of Clinical Microbiology and Infectious Diseases, 2017, 23 (3), pp.203-207 |
| بيانات النشر: | HAL CCSD, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Microbiology (medical), medicine.medical_specialty, Time Factors, Adolescent, Cystic Fibrosis, [SDV]Life Sciences [q-bio], 030106 microbiology, Context (language use), Biology, Real-Time Polymerase Chain Reaction, medicine.disease_cause, Sensitivity and Specificity, Gastroenterology, Cystic fibrosis, Microbiology, 03 medical and health sciences, Interquartile range, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Internal medicine, medicine, Humans, Pseudomonas Infections, Clinical significance, Prospective Studies, Child, Prospective cohort study, ComputingMilieux_MISCELLANEOUS, Bacteriological Techniques, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Pseudomonas aeruginosa, Sputum, General Medicine, medicine.disease, 3. Good health, [SDV] Life Sciences [q-bio], Early Diagnosis, Infectious Diseases, Real-time polymerase chain reaction, Molecular Diagnostic Techniques, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Female, medicine.symptom, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
| الوصف: | Objectives Early detection of Pseudomonas aeruginosa lung positivity is a key element in cystic fibrosis (CF) management. PCR has increased the accuracy of detection of many microorganisms. Clinical relevance of P. aeruginosa quantitative PCR (qPCR) in this context is unclear. Our aim was to determine P. aeruginosa qPCR sensitivity and specificity, and to assess the possible time saved by qPCR in comparison with standard practice (culture). Methods A multicentre cohort study was conducted over a 3-year period in 96 patients with CF without chronic P. aeruginosa colonization. Sputum samples were collected at each visit. Conventional culture and two-step qPCR ( opr L qPCR and gyr B /ecf X qPCR) were performed for 707 samples. The positivity criteria were based on the qPCR results, defined in a previous study as follow: opr L qPCR positivity alone if bacterial density was opr L qPCR combined with gyr B /ecf X qPCR if bacterial density was ≥730 CFU/mL. Results During follow up, 36 of the 96 patients with CF were diagnosed on culture as colonized with P. aeruginosa. This two-step qPCR displayed a sensitivity of 94.3% (95% CI 79.7%–98.6%), and a specificity of 86.3% (95% CI 83.4%–88.7%). It enabled P. aeruginosa acquisition to be diagnosed earlier in 20 patients, providing a median detection time gain of 8 months (interquartile range 3.7–17.6) for them. Conclusions Implementing opr L and gyr B /ecf X qPCR in the management of patients with CF allowed earlier detection of first P. aeruginosa lung positivity than culture alone. |
| اللغة: | English |
| تدمد: | 1198-743X 1469-0691 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b5f414f78134eef6ecc0918ba4e4cb0 https://hal.univ-brest.fr/hal-02048694 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....7b5f414f78134eef6ecc0918ba4e4cb0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1198743X 14690691 |
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