Sperm degradation after vasectomy follows a sperm chromatin fragmentation-dependent mechanism causing DNA breaks in the toroid linker regions
| العنوان: | Sperm degradation after vasectomy follows a sperm chromatin fragmentation-dependent mechanism causing DNA breaks in the toroid linker regions |
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| المؤلفون: | Jordi Ribas-Maynou, Hieu Nguyen, Raquel Valle, Hongwen Wu, Marc Yeste, W Steven Ward |
| المصدر: | Molecular Human Reproduction, 2022, vol. 28, núm. 9, p. gaac029 Articles publicats (D-B) Ribas Maynou, Jordi Nguyen, Hieu Valle, Raquel Wu, Hongwen Yeste Oliveras, Marc Ward, W.Steven 2022 Sperm degradation after vasectomy follows a sperm chromatin fragmentation dependent mechanism causing DNA breaks in the toroid linker regions Molecular Human Reproduction 28 9 gaac029 DUGiDocs – Universitat de Girona instname |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Epididymis, Male, Embryology, ADN -- Dany, Vasovasostomy, DNA Breaks, Obstetrics and Gynecology, Vasovasostomia, Cell Biology, DNA, Spermatozoa, Chromatin, Cromatina, Espermatozoides, Mice, Vas Deferens, Reproductive Medicine, Semen, Vasectomy, Genetics, DNA damage, Animals, Molecular Biology, Developmental Biology |
| الوصف: | Vasectomy is a widely used surgical technique creating an obstructive azoospermia. Although sperm cannot be ejaculated, the testis maintains sperm production in vasectomized males. The continuous accumulation of sperm deposited in the epididymis and the vas deferens fraction necessarily need to be degraded and eliminated. While the elimination process is carried out by granulomas that form after vasectomy, the detailed mechanisms of sperm degradation are still not known. The aim was to assess whether sperm chromatin fragmentation (SCF), a mechanism that degrades the entire sperm genome at the toroid linker regions (TLR), is activated after vasectomy in sperm cells. We vasectomized mice and evaluated the presence of TLR-specific double-strand breaks through pulsed-field gel electrophoresis and the Comet assay at 1, 2 and 3 weeks after surgery. Results for DNA damage (Olive tail moment) at single-cell level showed an increase of double-strand breaks after vasectomy for vas deferens sperm after 1, 2 and 3 weeks post-vasectomy (21.78 ± 2.29; 19.71 ± 1.79 and 32.59 ± 1.81, respectively), compared to mock surgery (7.04 ± 1.03; 10.10 ± 1.29 and 8.64 ± 0.85, respectively; P 0.05). Pulsed-field gel electrophoresis showed the presence of double-stranded breaks between 15 kb and 145 kb, indicating that DNA breaks were produced mainly in the sperm TLRs. Results presented here suggest that SCF is a mechanism activated in vas deferens after vasectomy to degrade sperm DNA when they cannot be ejaculated, preventing their function This research was supported by the European Union’s Horizon 2020 Research and Innovation Scheme under the Marie Skłodowska-Curie grant agreement No. 801342 (Tecniospring INDUSTRY; TECSPR-19- 1-0003), to J.R.-M.; the NIH, grant number GM123048 to W.S.W.; the Catalan Agency for Management of University and Research Grants, Regional Government of Catalonia, Spain (2017-SGR-1229); the Catalan Institution for Research and Advanced Studies (ICREA); and La Marató de TV3 Foundation (214/857-202039) |
| وصف الملف: | application/pdf |
| تدمد: | 1460-2407 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b71b113f0009fbb696803a171b0308d https://pubmed.ncbi.nlm.nih.gov/35929777 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....7b71b113f0009fbb696803a171b0308d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14602407 |
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