A germline-limited piggyBac transposase gene is required for precise excision in Tetrahymena genome rearrangement
| العنوان: | A germline-limited piggyBac transposase gene is required for precise excision in Tetrahymena genome rearrangement |
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| المؤلفون: | Guangying Wang, Mina Juma, Lifang Feng, Wei Miao, Arjune Dhanekula, Eduardo Orias, Wen Dui, Kai Chen, Amber Plemens, Yifan Liu, Jie Xiong, Xiao Chen, Hung Quang Dang, Eileen P. Hamilton, Lara Khadr, Geoffrey M. Kapler, Guanxiong Yan |
| المصدر: | Nucleic Acids Research |
| بيانات النشر: | Oxford University Press, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Protozoan Proteins, Transposases, Genome, Chromosomes, Tetrahymena thermophila, 03 medical and health sciences, Heterochromatin, Macronucleus, Genetics, Gene, Transposase, Gene Rearrangement, biology, Inverted Repeat Sequences, Tetrahymena, Gene rearrangement, Genomics, Exons, biology.organism_classification, 030104 developmental biology, RNA Interference, Programmed DNA elimination, Chromosome breakage, Micronucleus, Germline |
| الوصف: | Developmentally programmed genome rearrangement accompanies differentiation of the silent germline micronucleus into the transcriptionally active somatic macronucleus in the ciliated protozoan Tetrahymena thermophila. Internal eliminated sequences (IES) are excised, followed by rejoining of MAC-destined sequences, while fragmentation occurs at conserved chromosome breakage sequences, generating macronuclear chromosomes. Some macronuclear chromosomes, referred to as non-maintained chromosomes (NMC), are lost soon after differentiation. Large NMC contain genes implicated in development-specific roles. One such gene encodes the domesticated piggyBac transposase TPB6, required for heterochromatin-dependent precise excision of IES residing within exons of functionally important genes. These conserved exonic IES determine alternative transcription products in the developing macronucleus; some even contain free-standing genes. Examples of precise loss of some exonic IES in the micronucleus and retention of others in the macronucleus of related species suggest an evolutionary analogy to introns. Our results reveal that germline-limited sequences can encode genes with specific expression patterns and development-related functions, which may be a recurring theme in eukaryotic organisms experiencing programmed genome rearrangement during germline to soma differentiation. |
| اللغة: | English |
| تدمد: | 1362-4962 0305-1048 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7baa44ba95bb360a0c29d269ada18a86 http://europepmc.org/articles/PMC5766162 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....7baa44ba95bb360a0c29d269ada18a86 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13624962 03051048 |
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