Simple determination of hydrochlorothiazide in human plasma and urine by high performance liquid chromatography
| العنوان: | Simple determination of hydrochlorothiazide in human plasma and urine by high performance liquid chromatography |
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| المؤلفون: | Andreas Voss, J. X. de Vries |
| المصدر: | Biomedical Chromatography. 7:12-14 |
| بيانات النشر: | Wiley, 1993. |
| سنة النشر: | 1993 |
| مصطلحات موضوعية: | Adult, Male, medicine.medical_treatment, Clinical Biochemistry, Urine, Biochemistry, High-performance liquid chromatography, Analytical Chemistry, Hydrochlorothiazide, Drug Discovery, medicine, Humans, Molecular Biology, Chromatography, High Pressure Liquid, Pharmacology, Detection limit, Chromatography, Hydroflumethiazide, Elution, Chemistry, General Medicine, Reversed-phase chromatography, Spectrophotometry, Ultraviolet, Diuretic, medicine.drug |
| الوصف: | The diuretic drug hydrochlorothiazide (HCT) is used mainly for treatment of mild to moderate hypertension and is usually administered with other drugs. An assay for the determination of HCT in human plasma and urine by high performance liquid chromatography (HPLC) has been developed. Samples were purified by solvent extraction and analysed by reversed phase HPLC with ultraviolet detection, using hydroflumethiazide as the internal standard; plasma was eluted using gradient elution and urine was analysed isocratically. The method is simple to perform, is sensitive (detection limit 0.01 micrograms/mL in plasma and 0.2 micrograms/mL for urine); it showed good reproducibility (3-8%). A great number of drugs did not interfere with the assay and the method was used for pharmacokinetic studies in healthy subjects, but samples from patients can also be analysed with high selectivity. |
| تدمد: | 1099-0801 0269-3879 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7bbb8f26f1cf3a9c72cde3ceeb1c1d37 https://doi.org/10.1002/bmc.1130070104 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....7bbb8f26f1cf3a9c72cde3ceeb1c1d37 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10990801 02693879 |
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