Identification of Subunits for Novel Universal Vaccines against Three Predominant Serogroups and the Emerging O145 among Avian Pathogenic Escherichia coli by Pan-RV Pipeline
| العنوان: | Identification of Subunits for Novel Universal Vaccines against Three Predominant Serogroups and the Emerging O145 among Avian Pathogenic Escherichia coli by Pan-RV Pipeline |
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| المؤلفون: | Zhuohao Wang, Sixiang Xu, Xiangkuan Zheng, Xiaofeng Zheng, Meihan Liu, Genglin Guo, Yanfei Yu, Xiangan Han, Yuqing Liu, Kaicheng Wang, Wei Zhang |
| المصدر: | Appl Environ Microbiol |
| بيانات النشر: | American Society for Microbiology, 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Ecology, Methods, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| الوصف: | Avian pathogenic Escherichia coli, a causative agent of avian colibacillosis, has been causing serious economic losses in the poultry industry. The increase in multidrug-resistant isolates and the complexity of the serotypes of this pathogen, especially the recently reported emergence of a newly predominant serogroup of O145, make the control of this disease difficult. To address this challenge, a high-throughput screening approach, called Pan-RV (Reverse vaccinology based on pangenome analysis), is proposed to search for universal protective antigens against the three traditional serogroups and the newly emerged O145. Using this approach, a total of 61 proteins regarded as probable antigens against the four important serogroups were screened from the core genome of 127 Avian pathogenic Escherichia coli (APEC) genomes, and six were verified by Western blots using antisera. Overall, our research will provide a foundation for the development of an APEC subunit vaccine against avian colibacillosis. Given the exponential growth of whole-genome sequencing (WGS) data, our Pan-RV pipeline will make screening of bacterial vaccine candidates inexpensive, rapid, and efficient. IMPORTANCE With the emergence of drug resistance and the newly predominant serogroup O145, the control of Avian pathogenic Escherichia coli is facing a serious challenge; an efficient immunological method is urgently needed. Here, for the first time, we propose a high-throughput screening approach to search for universal protective antigens against the three traditional serogroups and the newly emerged O145. Importantly, using this approach, a total of 61 proteins regarded as probable antigens against the four important serogroups were screened, and three were shown to be immunoreactive with all antisera (covering the four serogroups), thereby providing a foundation for the development of APEC subunit vaccines against avian colibacillosis. Further, our Pan-RV pipeline will provide immunological control strategies for pathogens with complex and variable genetic backgrounds such as Escherichia coli and will make screening of bacterial vaccine candidates more inexpensive, rapid, and efficient. |
| تدمد: | 1098-5336 0099-2240 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7bdde944e5521a774a3aeca9e0da1159 https://doi.org/10.1128/aem.01061-22 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....7bdde944e5521a774a3aeca9e0da1159 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985336 00992240 |
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