IL17A critically shapes the transcriptional program of fibroblasts in pancreatic cancer and switches on their protumorigenic functions
| العنوان: | IL17A critically shapes the transcriptional program of fibroblasts in pancreatic cancer and switches on their protumorigenic functions |
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| المؤلفون: | Pavol Lukáč, Roberto Chiarle, Aldo Scarpa, Claudia Curcio, Michela Capello, Wanda Y. Li, Luca Vannucci, Gianluca Mucciolo, Roberta Curto, Dmitry Stakheev, Maria Antonietta Satolli, Paola Cappello, Paolo Provero, Rita T. Lawlor, Francesco Novelli, Cecilia Roux, Daniele Giordano, Tak W. Mak |
| المصدر: | Proc Natl Acad Sci U S A |
| بيانات النشر: | Proceedings of the National Academy of Sciences, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Stromal cell, Carcinogenesis, cancer-associated fibroblast, extracellular matrix, medicine.medical_treatment, T cell, pancreatic cancer, Adenocarcinoma, CD8-Positive T-Lymphocytes, Mice, Immune system, Cancer-Associated Fibroblasts, Tumor Microenvironment, medicine, Animals, Humans, Mice, Knockout, Tumor microenvironment, Multidisciplinary, Chemistry, IL17A | pancreatic cancer | cancer-associated fibroblast | extracellular matrix | fibrosis, fibrosis, Interleukin-17, FOXP3, Forkhead Transcription Factors, Receptors, Interleukin, Biological Sciences, Disease Models, Animal, Cytokine, medicine.anatomical_structure, IL17A, Cancer research, CD80, CD8, Carcinoma, Pancreatic Ductal |
| الوصف: | A hallmark of cancer, including pancreatic ductal adenocarcinoma (PDA), is a massive stromal and inflammatory reaction. Many efforts have been made to identify the anti- or protumoral role of cytokines and immune subpopulations within the stroma. Here, we investigated the role of interleukin-17A (IL17A) and its effect on tumor fibroblasts and the tumor microenvironment. We used a spontaneous PDA mouse model (KPC) crossed to IL17A knockout mice to show an extensive desmoplastic reaction, without impaired immune infiltration. Macrophages, especially CD80(+) and T cells, were more abundant at the earlier time point. In T cells, a decrease in FoxP3(+) cells and an increase in CD8(+) T cells were observed in KPC/IL17A(−/−) mice. Fibroblasts isolated from IL17A(+/+) and IL17A(−/−) KPC mice revealed very different messenger RNA (mRNA) and protein profiles. IL17A(−/−) fibroblasts displayed the ability to restrain tumor cell invasion by producing factors involved in extracellular matrix remodeling, increasing T cell recruitment, and producing higher levels of cytokines and chemokines favoring T helper 1 cell recruitment and activation and lower levels of those recruiting myeloid/granulocytic immune cells. Single-cell quantitative PCR on isolated fibroblasts confirmed a very divergent profile of IL17A-proficient and -deficient cells. All these features can be ascribed to increased levels of IL17F observed in the sera of IL17A(−/−) mice, and to the higher expression of its cognate receptor (IL17RC) specifically in IL17A(−/−) cancer-associated fibroblasts (CAFs). In addition to the known effects on neoplastic cell transformation, the IL17 cytokine family uniquely affects fibroblasts, representing a suitable candidate target for combinatorial immune-based therapies in PDA. |
| تدمد: | 1091-6490 0027-8424 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7bf2631e4c2c899cf8a5d98635d991c5 https://doi.org/10.1073/pnas.2020395118 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....7bf2631e4c2c899cf8a5d98635d991c5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10916490 00278424 |
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