The proteomic landscape of small urinary extracellular vesicles during kidney transplantation
| العنوان: | The proteomic landscape of small urinary extracellular vesicles during kidney transplantation |
|---|---|
| المؤلفون: | Victor G. Puelles, Katrin Bohl, Roger Wahba, Jan-Wilm Lackmann, Christine Kurschat, Daniel Bachurski, Oliver Kretz, Dirk L. Stippel, Bernhard Schermer, Fabian Braun, Tobias B. Huber, Thomas Benzing, Markus M. Rinschen, Michael Hallek, Roman-Ulrich Müller, Heike Göbel, Martin Richard Späth, Corinna Klein, Andreas Beyer, Philipp Antczak, Denise Buchner, Ingo Plagmann |
| المصدر: | Braun, F, Rinschen, M, Buchner, D, Bohl, K, Plagmann, I, Bachurski, D, Richard Späth, M, Antczak, P, Göbel, H, Klein, C, Lackmann, J-W, Kretz, O, Puelles, V G, Wahba, R, Hallek, M, Schermer, B, Benzing, T, Huber, T B, Beyer, A, Stippel, D, Kurschat, C E & Müller, R-U 2020, ' The proteomic landscape of small urinary extracellular vesicles during kidney transplantation ', Journal of Extracellular Vesicles, vol. 10, no. 1, e12026 . https://doi.org/10.1002/jev2.12026 Journal of Extracellular Vesicles |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Adult, Male, Proteomics, transplant biology, Histology, Urinary system, medicine.medical_treatment, kidney transplantation, Biology, Bioinformatics, 03 medical and health sciences, Extracellular Vesicles, 0302 clinical medicine, medicine, Living Donors, Humans, complement, Renal replacement therapy, urinary extracellular vesicles, Kidney transplantation, Research Articles, Aged, Cell Biology, Extracellular vesicle, Middle Aged, medicine.disease, Allografts, Prognosis, Transplantation, 030104 developmental biology, 030220 oncology & carcinogenesis, Proteome, Biomarker (medicine), biomarker, Female, Biomarkers, Phosphoenolpyruvate Carboxykinase (ATP), chronic kidney disease, Research Article |
| الوصف: | Kidney transplantation is the preferred renal replacement therapy available. Yet, long‐term transplant survival is unsatisfactory, partially due to insufficient possibilities of longitudinal monitoring and understanding of the biological processes after transplantation. Small urinary extracellular vesicles (suEVs) – as a non‐invasive source of information – were collected from 22 living donors and recipients. Unbiased proteomic analysis revealed temporal patterns of suEV protein signature and cellular processes involved in both early response and longer‐term graft adaptation. Complement activation was among the most dynamically regulated components. This unique atlas of the suEV proteome is provided through an online repository allowing dynamic interrogation by the user. Additionally, a correlative analysis identified putative prognostic markers of future allograft function. One of these markers – phosphoenol pyruvate carboxykinase (PCK2) – could be confirmed using targeted MS in an independent validation cohort of 22 additional patients. This study sheds light on the impact of kidney transplantation on urinary extracellular vesicle content and allows the first deduction of early molecular processes in transplant biology. Beyond that our data highlight the potential of suEVs as a source of biomarkers in this setting. |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7c5d4e041c62f9ea4c77c63efc06a144 https://pure.au.dk/portal/da/publications/the-proteomic-landscape-of-small-urinary-extracellular-vesicles-during-kidney-transplantation(0bfb4f79-111a-44b8-9270-e682dd23edfb).html |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....7c5d4e041c62f9ea4c77c63efc06a144 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |