Figure Legend and Supplementary Figure 1 from Differential Immune Microenvironments and Response to Immune Checkpoint Blockade among Molecular Subtypes of Murine Medulloblastoma
التفاصيل البيبلوغرافية
العنوان:
Figure Legend and Supplementary Figure 1 from Differential Immune Microenvironments and Response to Immune Checkpoint Blockade among Molecular Subtypes of Murine Medulloblastoma
American Association for Cancer Research (AACR), 2023.
سنة النشر:
2023
الوصف:
Orthotopically transplanted tumors are uniformly lethal at determined minimum tumorigenic dose. (A) Tumors arising from Patched1 heterozygous (Ptch1+/-) mice and immune deficient NOD scid gamma (NSG) mice implanted with retrovirally transduced cerebellar neural stem cells (NSC) were serially passaged via stereotactic implantation in the cerebellum of immunocompetent C57BL/6 hosts. Animals were implanted with varying numbers of tumor cells to determine the minimum dose required to induce 100% lethality in tumor-bearing mice within one month. Stereotactic implantation of (B) 1 x 103 NSC MB tumor cells and (C) 1.25 x 105 Ptch1 MB cells were determined as the minimum tumorigenic dose carried forth in subsequent animal studies.
