Effects of Formalin-Inactivated Respiratory Syncytial Virus (FI-RSV) in the Perinatal Lamb Model of RSV
| العنوان: | Effects of Formalin-Inactivated Respiratory Syncytial Virus (FI-RSV) in the Perinatal Lamb Model of RSV |
|---|---|
| المؤلفون: | Steven M. Varga, Albert Van Geelen, Mark R. Ackermann, Rachel J. Derscheid, Jack M. Gallup, Drew D. Grosz, Cory J. Knudson, Shannon J. Hostetter |
| المصدر: | PLoS ONE PLoS ONE, Vol 8, Iss 12, p e81472 (2013) |
| بيانات النشر: | Public Library of Science, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | viruses, Plasma Cells, lcsh:Medicine, Respiratory Syncytial Virus Infections, Biology, Virus, 03 medical and health sciences, 0302 clinical medicine, Immune system, Neutralization Tests, Formaldehyde, medicine, Animals, Humans, Respiratory system, lcsh:Science, Antigens, Viral, Lung, Sheep, Domestic, 030304 developmental biology, 0303 health sciences, Multidisciplinary, medicine.diagnostic_test, lcsh:R, Vaccination, virus diseases, respiratory system, medicine.disease, Virology, Immunohistochemistry, 3. Good health, Respiratory Syncytial Viruses, Titer, Disease Models, Animal, Bronchoalveolar lavage, medicine.anatomical_structure, Animals, Newborn, Bronchiolitis, 030220 oncology & carcinogenesis, Immunology, RNA, Viral, Virus Inactivation, lcsh:Q, Bronchoalveolar Lavage Fluid, Research Article |
| الوصف: | Respiratory syncytial virus (RSV) is the most frequent cause of bronchiolitis in infants and children worldwide. There are currently no licensed vaccines or effective antivirals. The lack of a vaccine is partly due to increased caution following the aftermath of a failed clinical trial of a formalin-inactivated RSV vaccine (FI-RSV) conducted in the 1960’s that led to enhanced disease, necessitating hospitalization of 80% of vaccine recipients and resulting in two fatalities. Perinatal lamb lungs are similar in size, structure and physiology to those of human infants and are susceptible to human strains of RSV that induce similar lesions as those observed in infected human infants. We sought to determine if perinatal lambs immunized with FI-RSV would develop key features of vaccine-enhanced disease. This was tested in colostrum-deprived lambs immunized at 3–5 days of age with FI-RSV followed two weeks later by RSV infection. The FI-RSV-vaccinated lambs exhibited several key features of RSV vaccine-enhanced disease, including reduced RSV titers in bronchoalveolar lavage fluid and lung, and increased infiltration of peribronchiolar and perivascular lymphocytes compared to lambs either undergoing an acute RSV infection or naive controls; all features of RSV vaccine-enhanced disease. These results represent a first step proof-of-principle demonstration that the lamb can develop altered responses to RSV following FI-RSV vaccination. The lamb model may be useful for future mechanistic studies as well as the assessment of RSV vaccines designed for infants. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7cc36f58ca70d48853221c01139beb26 http://europepmc.org/articles/PMC3855688 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....7cc36f58ca70d48853221c01139beb26 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
|---|