The intestinal barrier in irritable bowel syndrome: subtype-specific effects of the systemic compartment in an in vitro model
| العنوان: | The intestinal barrier in irritable bowel syndrome: subtype-specific effects of the systemic compartment in an in vitro model |
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| المؤلفون: | Samefko Ludidi, Ad A.M. Masclee, Elhaseen Elamin, José M. Conchillo, Harm-Jan Pieters, Joanna W. Kruimel, Paul Bours, Esther Schaepkens, Daisy Jonkers |
| المساهمون: | RS: NUTRIM - R2 - Gut-liver homeostasis, Toxicogenomics, Interne Geneeskunde, MUMC+: MA Maag Darm Lever (9) |
| المصدر: | PLoS ONE PLOS ONE, 10(5):e0123498. Public Library of Science PLoS ONE, Vol 10, Iss 5, p e0123498 (2015) |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Lipopolysaccharides, medicine.medical_specialty, lcsh:Medicine, Tryptase, Irritable Bowel Syndrome, Internal medicine, Blood plasma, medicine, Humans, Intestinal Mucosa, lcsh:Science, Irritable bowel syndrome, Barrier function, Gastrointestinal tract, Multidisciplinary, Intestinal permeability, biology, Chemistry, lcsh:R, medicine.disease, Pathophysiology, Extracellular Matrix, Intestines, Endocrinology, Caco-2, Immunology, biology.protein, lcsh:Q, Tryptases, Caco-2 Cells, Research Article |
| الوصف: | BACKGROUND: Irritable bowel syndrome (IBS) is a disorder with multifactorial pathophysiology. Intestinal barrier may be altered, especially in diarrhea-predominant IBS (IBS-D). Several mediators may contribute to increased intestinal permeability in IBS. AIM: We aimed to assess effects of tryptase and LPS on in vitro permeability using a 3-dimensional cell model after basolateral cell exposure. Furthermore, we assessed the extent to which these mediators in IBS plasma play a role in intestinal barrier function. MATERIALS AND METHODS: Caco-2 cells were grown in extracellular matrix to develop into polarized spheroids and were exposed to tryptase (10 - 50 mU), LPS (1 - 50 ng/mL) and two-fold diluted plasma samples of 7 patients with IBS-D, 7 with constipation-predominant IBS (IBS-C) and 7 healthy controls (HC). Barrier function was assessed by the flux of FITC-dextran (FD4) using live cell imaging. Furthermore, plasma tryptase and LPS were determined. RESULTS: Tryptase (20 and 50 mU) and LPS (6.25 - 50 ng/mL) significantly increased Caco-2 permeability versus control (all P< 0.05). Plasma of IBS-D only showed significantly elevated median tryptase concentrations (7.1 [3.9 - 11.0] vs. 4.2 [2.2 - 7.0] vs. 4.2 [2.5 - 5.9] mug/mL; P |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7d39d5b8886d26767808228ac30ca955 https://pubmed.ncbi.nlm.nih.gov/25978614 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....7d39d5b8886d26767808228ac30ca955 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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