Berberine Protects Against Simulated Ischemia/Reperfusion Injury-Induced H9C2 Cardiomyocytes Apoptosis In Vitro and Myocardial Ischemia/Reperfusion-Induced Apoptosis In Vivo by Regulating the Mitophagy-Mediated HIF-1α/BNIP3 Pathway
| العنوان: | Berberine Protects Against Simulated Ischemia/Reperfusion Injury-Induced H9C2 Cardiomyocytes Apoptosis In Vitro and Myocardial Ischemia/Reperfusion-Induced Apoptosis In Vivo by Regulating the Mitophagy-Mediated HIF-1α/BNIP3 Pathway |
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| المؤلفون: | Yuwei Zhang, Li Li, Jiang Li, Jinying Li, Xueming Cao, Peiyuan Hao, Na Zhu, Qiubo Du, Yongli Li |
| المصدر: | Frontiers in Pharmacology, Vol 11 (2020) Frontiers in Pharmacology |
| بيانات النشر: | Frontiers Media S.A., 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Pharmacology, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Berberine, In vivo, berberine, Mitophagy, myocardial reperfusion injury, HIF-1α/BNIP3 pathway, medicine, Pharmacology (medical), Original Research, Gene knockdown, Chemistry, Cell growth, lcsh:RM1-950, myocardial ischemia injury, medicine.disease, In vitro, 030104 developmental biology, mitophagy, lcsh:Therapeutics. Pharmacology, Apoptosis, 030220 oncology & carcinogenesis, Reperfusion injury |
| الوصف: | Berberine (BBR) has a variety of pharmacological activities and is widely used in Asian countries. However, the clinical application of BBR still lacks scientific basis, what protective mechanism of BBR against myocardial ischemia-reperfusion injury (MIRI). In vitro experiments, BBR pretreatment regulated autophagy-related protein expression, induced cell proliferation and autophagosome formation, and reduced the mitochondrial membrane potential (ΔΨm) increase in H9C2 cells. In vivo experiments, BBR reduced the myocardial infarct size, decreased cardiomyocyte apoptosis, and markedly decreased myocardial enzyme (CK-MB, LDH, and AST) activity-induced I/R. In addition, upon BNIP3 knockdown, the regulatory effects of BBR on the above indicators were weakened both in H9C2 cells and in vivo. Luciferase reporter and ChIP assays indicated that BBR mediated BNIP3 expression by enhancing the binding of HIF-1α to the BNIP3 promoter. BBR protects against myocardial I/R injury by inducing cardiomyocytes proliferation, inhibiting cardiomyocytes apoptosis, and inducing the mitophagy-mediated HIF-1α/BNIP3 pathway. Thus, BBR may serve as a novel therapeutic drug for myocardial I/R injury. |
| اللغة: | English |
| تدمد: | 1663-9812 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7dcbcbf3041c4b908ac01a9a35fa9c78 https://www.frontiersin.org/article/10.3389/fphar.2020.00367/full |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....7dcbcbf3041c4b908ac01a9a35fa9c78 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16639812 |
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