Distribution of human colonic dendritic cells and macrophages
| العنوان: | Distribution of human colonic dendritic cells and macrophages |
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| المؤلفون: | William F. Doe, Lesley Maxwell, E van de Pol, Paul Pavli |
| المصدر: | Clinical and Experimental Immunology. 104:124-132 |
| بيانات النشر: | Oxford University Press (OUP), 1996. |
| سنة النشر: | 1996 |
| مصطلحات موضوعية: | Lamina propria, Follicular dendritic cells, Colon, Macrophages, T cell, Immunoelectron microscopy, CD14, Immunology, Original Articles, Cell Separation, Dendritic Cells, Dendritic cell, Biology, Immunohistochemistry, Immunophenotyping, medicine.anatomical_structure, Cell Adhesion, medicine, Humans, Immunology and Allergy, Macrophage, Intestinal Mucosa, Antigen-presenting cell |
| الوصف: | SUMMARY To define the phenotype of intestinal dendritic cells and macrophages, resected colonic specimens were used to obtain lamina propria cell suspensions by EDTA treatment, then enzymatic digestion. The phenotype of dendritic cell-enriched suspensions was compared with that of macrophage-enriched populations by immunocytochemistry using the avidin-biotin-peroxidase (ABC) system and immunoelectron microscopy. Dendritic cells expressed HLA-DR (L243) and HLA-DQ-associated (RFD1) antigens and CD68 in a perinuclear distribution. Staining for S100 was weak or absent. Macrophages also expressed HLA markers (L243 and RFD1) and CD68. The 25F9 antigen was expressed strongly, whilst CD14 was absent from cells isolated from non-inflamed tissues. To determine their anatomic distribution, immunohistochemistry was performed using single- and double-labelling techniques (ABC ± alkaline phosphatase anti-alkaline phosphatase method). Mutually exclusive subsets of 25F9+ and S100+cells were seen: 25F9+ macrophages were concentrated in a band immediately beneath the luminal epithelium; S100+/HLA-DR+ dendritic cells formed a reticular network throughout the lamina propria and beneath the basement membrane of the crypts. This distribution suggests that macrophages may help regulate intestinal responses by acting as the first line of defence against the entry of luminal antigens. A breach of the macrophage ‘barrier’ by invading antigens may necessitate the recruitment of T cell responses by immunostimulatory dendritic cells. |
| تدمد: | 1365-2249 0009-9104 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7de154227dd0e5a5cc9f6b8b1cc6cc02 https://doi.org/10.1046/j.1365-2249.1996.d01-642.x |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....7de154227dd0e5a5cc9f6b8b1cc6cc02 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13652249 00099104 |
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