Psychrotrophic properties, toxigenic characteristics, and PFGE profiles of Bacillus cereus isolated from different foods and spices
| العنوان: | Psychrotrophic properties, toxigenic characteristics, and PFGE profiles of Bacillus cereus isolated from different foods and spices |
|---|---|
| المؤلفون: | Alper Karagöz, Hüseyin Burak Dişli, Hayriye Yeşim Can, Mehmet Elmalı |
| المصدر: | Ciência Rural v.52 n.4 2022 Ciência Rural Universidade Federal de Santa Maria (UFSM) instacron:UFSM Ciência Rural, Vol 52, Iss 4 (2021) |
| بيانات النشر: | Universidade Federal de Santa Maria, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Agriculture (General), Food spoilage, Bacillus cereus, Biology, S1-972, medicine, Pulsed-field gel electrophoresis, Food science, PFGE typing, Food poisoning, General Veterinary, fungi, Hemolysin, Agriculture, Raw milk, medicine.disease, biology.organism_classification, toxin gene, PCR, Cereus, food poisoning, Animal Science and Zoology, Agronomy and Crop Science, Bacteria |
| الوصف: | Bacillus cereus is an aerobic and facultatively anaerobic, spore-forming bacterium, and it is found naturally in soil and poses a risk factor for the contamination of food and foodstuffs including cereals, vegetables, spices, ready-to-eat (RTE) foods, meats, milk, and dairy products. This study determined the prevalence of B. cereus in raw poultry meat, raw cow’s milk, cheese, spices, and RTE foods in Hatay province. The study also analysed the psychrotrophic properties, toxigenic characteristics, and pulsed-field gel electrophoresis (PFGE) profiles of the isolates. The levels of contamination with B. cereus determined for cheese, raw milk, RTE foods, spices, and raw poultry meat were 16.6%, 34.2%, 42.8%, 49%, and 55.5%, respectively. B. cereus was isolated from 84 (42%) of the 200 samples analysed and the 84 isolates were verified by PCR analysis targeting the haemolysin gene specific for B. cereus. Of the total isolates, 64 (76.1%) were psychrotrophic. The toxin gene profiling of B. cereus isolates was determined by amplifying the four genes nhe, hbl, cytK, and ces. The nhe and cytK genes were most frequently detected in the isolates, while the hbl and ces genes were not found. In addition, a high genetic relationship between the isolates was detected at a 92% similarity level by PFGE analysis. In conclusion, the occurrence of both psychrotrophic and toxigenic B. cereus strains in this study indicated a potential risk for food spoilage and food poisoning. |
| وصف الملف: | text/html |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7de286d047830c41e6cee4b9b2286819 http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782022000400753 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....7de286d047830c41e6cee4b9b2286819 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |