Lysostaphin eradicates established Staphylococcus aureus biofilms in jugular vein catheterized mice
| العنوان: | Lysostaphin eradicates established Staphylococcus aureus biofilms in jugular vein catheterized mice |
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| المؤلفون: | James J. Mond, Tanya Chanturiya, John F. Kokai-Kun |
| المصدر: | Journal of Antimicrobial Chemotherapy. 64:94-100 |
| بيانات النشر: | Oxford University Press (OUP), 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Microbiology (medical), Catheterization, Central Venous, Staphylococcus aureus, Micrococcaceae, medicine.drug_class, Antibiotics, Colony Count, Microbial, Biology, medicine.disease_cause, Microbiology, Nafcillin, Mice, Jugular vein, medicine, Animals, Humans, Pharmacology (medical), Antibacterial agent, Pharmacology, Lysostaphin, Heart, Staphylococcal Infections, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, Anti-Bacterial Agents, Catheter, Infectious Diseases, Equipment and Supplies, Liver, Biofilms, Jugular Veins, medicine.drug |
| الوصف: | Objectives: Staphylococcus aureus infections associated with indwelling devices can be very difficult to treat due to the recalcitrant nature of bacterial biofilms to conventional antibiotics. Lysostaphin has been shown to clear S. aureus biofilms in vitro, and in this study we determined whether lysostaphin could also eradicate established S. aureus biofilms on implanted jugular vein catheters in mice. Methods: Jugular vein catheterized mice (four to six per group) challenged with S. aureus developed multiorgan infection and biofilm infections on the catheters. The infected mice with established biofilms received various doses of recombinant lysostaphin through the catheters, administered up to three times daily for up to 4 days. Some mice also received lysostaphin combined with nafcillin. Following treatment, mice were sacrificed and cfu on the catheter and in the liver and heart were determined. In another set of experiments, implanted jugular vein catheters in mice were pre-instilled with lysostaphin to determine whether this pre-treatment would protect the mice from biofilm infection. Results and conclusions: Lysostaphin administered at 15 mg/kg in combination with 50 mg/kg nafcillin three times per day for 4 days eradicated established S. aureus, including methicillin-resistant S. aureus, biofilms from implanted catheters and sterilized heart and liver infections of S. aureusinfected mice. Furthermore, a single pre-instillation of 10 mg/kg lysostaphin in catheters completely protected catheterized mice from a subsequent biofilm infection. These results demonstrate that lysostaphin is an effective treatment as well as prophylaxis for S. aureus biofilms on indwelling catheters. |
| تدمد: | 1460-2091 0305-7453 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7e06fdedbbddc789d02931ac5a287d17 https://doi.org/10.1093/jac/dkp145 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....7e06fdedbbddc789d02931ac5a287d17 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14602091 03057453 |
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