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Trends in chloroquine resistance marker, Pfcrt-K76T mutation ten years after chloroquine withdrawal in Tanzania

التفاصيل البيبلوغرافية
العنوان: Trends in chloroquine resistance marker, Pfcrt-K76T mutation ten years after chloroquine withdrawal in Tanzania
المؤلفون: Akili K. Kalinga, Asia Mohammed, Jackline F Mosha, Alphaxard Manjurano, Cally Roper, Hugh Reyburn, Dominick F Mosha, Michael Alifrangis, Reginald A. Kavishe, Frank W. Mosha, Marco van Zwetselaar, Jan B. Koenderink, Arnold Ndaro
المصدر: Malaria Journal
Malaria Journal, 12, 415
Malaria Journal, 12, 1, pp. 415
سنة النشر: 2013
مصطلحات موضوعية: Male, Genotyping Techniques, Drug Resistance, Protozoan Proteins, Drug resistance, Polymerase Chain Reaction, Tanzania, 0302 clinical medicine, Chloroquine, Pregnancy, Genotype, Artemisinin, Child, GeneralLiterature_REFERENCE(e.g.,dictionaries,encyclopedias,glossaries), Diagnosis & treatment, 0303 health sciences, biology, Pfcrt, Middle Aged, 3. Good health, Infectious Diseases, Child, Preschool, Female, Polymorphism, Restriction Fragment Length, Mutations, medicine.drug, Adult, medicine.medical_specialty, Adolescent, 030231 tropical medicine, Plasmodium falciparum, 03 medical and health sciences, Antimalarials, Young Adult, Internal medicine, parasitic diseases, medicine, Humans, Point Mutation, Parasites, Aged, 030306 microbiology, Research, Infant, Newborn, Infant, Membrane Transport Proteins, DNA, Protozoan, medicine.disease, biology.organism_classification, Malaria, Parasitology, Immunology, Polymorphisms, Membrane transport and intracellular motility Poverty-related infectious diseases [NCMLS 5]
الوصف: Background Plasmodium falciparum resistance to anti-malarial drugs remains a major obstacle to the control of malaria. In 2001 Tanzania replaced chloroquine (CQ) with sulphadoxine-pyrimethamine (SP) as first-line drug, which in turn was replaced by artemisinin combination therapy in 2006. SP has however, continued to be used in intermittent preventive treatment of malaria in pregnancy (IPTp) despite reports of high levels of resistance to SP due to the lack of alternatives to SP for IPTp. Recent reports have indicated recovery of CQ-susceptibility in Malawi, Kenya, Mozambique, and Tanzania based on the prevalence of wild types at codon 76 of the Pfcrt gene in indigenous P. falciparum populations. The current prevalence of this Pfcrt- 76 CQ resistance marker from six regions of Tanzania mainland is hereby reported. Methods DNA extracted from filter-paper dried blood spots and rapid diagnostics kit strips collected from finger-prick blood were used to genotype the Pfcrt-76 resistance marker using PCR-RFLP. Data from previously published studies were used to generate CQ susceptibility recovery trends using logistic regression model. Results Seven hundred and forty one (741) samples were genotyped. The current frequency of the CQ-susceptible Pfcrt-K76 was above 92% and did not differ between regions in Tanzania (χ 2 = 2.37; p = 0.795). The K76 allelic prevalence was between 85.7 and 93% in regions (χ 2 = 7.88, p = 0.163). The CQ resistance recovery trends showed regional variability that may be caused by differences in malaria transmission intensity, but overall the trends converge as the susceptibility levels in all regions approach >90%. Conclusions CQ withdrawal in Tanzania has resulted into >90% recovery of susceptibility in ten years of withdrawal. These findings are in support of the search for CQ-based combination drugs as a possible future alternative to SP for IPTp in places where full recovery of CQ-susceptibility will be evident.
وصف الملف: application/pdf
تدمد: 1475-2875
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7f7bc36c68c25e635422dacdaff5c9f6
https://pubmed.ncbi.nlm.nih.gov/24225406
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....7f7bc36c68c25e635422dacdaff5c9f6
قاعدة البيانات: OpenAIRE
الوصف
تدمد:14752875