Light-triggered RNA release and induction of hMSC osteogenesis via photodegradable, dual-crosslinked hydrogels
| العنوان: | Light-triggered RNA release and induction of hMSC osteogenesis via photodegradable, dual-crosslinked hydrogels |
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| المؤلفون: | Cong Truc Huynh, Minh Khanh Nguyen, Mantas Naris, Gulen Yesilbag Tonga, Vincent M. Rotello, Eben Alsberg |
| المصدر: | Nanomedicine. 11:1535-1550 |
| بيانات النشر: | Future Medicine Ltd, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Materials science, Ultraviolet Rays, Cellular differentiation, Biomedical Engineering, Medicine (miscellaneous), Bioengineering, Nanotechnology, 02 engineering and technology, Development, Cell Line, HeLa, 03 medical and health sciences, chemistry.chemical_compound, Tissue engineering, Osteogenesis, microRNA, Humans, General Materials Science, RNA, Small Interfering, Polyethylenimine, Photolysis, Tissue Engineering, biology, technology, industry, and agriculture, RNA, Cell Differentiation, Hydrogels, Mesenchymal Stem Cells, 021001 nanoscience & nanotechnology, biology.organism_classification, Controlled release, MicroRNAs, 030104 developmental biology, chemistry, Delayed-Action Preparations, Self-healing hydrogels, Biophysics, 0210 nano-technology, Research Article, HeLa Cells |
| الوصف: | Aim: To engineer a photodegradable hydrogel system for actively controlled release of bioactive unmodified RNA at designated time points to induce hMSC osteogenesis. Materials & methods: RNA/polyethylenimine complexes were loaded into dual-crosslinked photodegradable hydrogels to examine the capacity of UV light application to trigger their release. The ability of released RNA to drive hMSC osteogenic differentiation was also investigated. Results & conclusion: RNA release from photodegradable hydrogels was accelerated upon UV application, which was not observed in non-photodegradable hydrogels. Regardless of the presence of UV light, released siGFP exhibited high bioactivity by silencing GFP expression in HeLa cells. Importantly, siNoggin or miRNA-20a released from the hydrogels induced hMSC osteogenesis. This system provides a potentially valuable physician/patient-controlled ‘on-demand’ RNA delivery platform for biomedical applications. |
| تدمد: | 1748-6963 1743-5889 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7fcb1c3bc94857467455c571e51fd3a4 https://doi.org/10.2217/nnm-2016-0088 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....7fcb1c3bc94857467455c571e51fd3a4 |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 17486963 17435889 |
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