Proteomic analysis of the monkey hippocampus for elucidating ischemic resistance
| العنوان: | Proteomic analysis of the monkey hippocampus for elucidating ischemic resistance |
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| المؤلفون: | Tetsumori Yamashima, Yuki Kitamura, Saeko Tada-Oikawa, Shinji Oikawa, Mariko Murata, Yurie Mori, Hatasu Kobayashi, Shota Kurimoto |
| المصدر: | Journal of Clinical Biochemistry and Nutrition |
| بيانات النشر: | The Society for Free Radical Research Japan, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, 030109 nutrition & dietetics, Nutrition and Dietetics, hippocampus, Chemistry, Dentate gyrus, Protein Carbonylation, Clinical Biochemistry, Medicine (miscellaneous), carbonylation, SIRT2, Protein oxidation, Neuroprotection, HSPA1A, Cell biology, 03 medical and health sciences, proteomics, 0302 clinical medicine, oxidative stress, Protein deacetylase, Original Article, 030211 gastroenterology & hepatology, dentate gyrus, NAD+ kinase |
| الوصف: | It is well-known that the cornu Ammonis 1 (CA1) sector of hippocampus is vulnerable for the ischemic insult, whereas the dentate gyrus (DG) is resistant. Here, to elucidate its underlying mechanism, alternations of protein oxidation and expression of DG in the monkey hippocampus after ischemia-reperfusion by the proteomic analysis were studied by comparing CA1 data. Oxidative damage to proteins such as protein carbonylation interrupt the protein function. Carbonyl modification of molecular chaperone, heat shock 70 kDa protein 1 (Hsp70.1) was increased remarkably in CA1, but slightly in DG. In addition, expression levels of nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylase sirtuin-2 (SIRT2) was significantly increased in DG after ischemia, but decreased in CA1. Accordingly, it is likely that SIRT2 upregulation and negligible changes of carbonylation of Hsp70.1 exert its neuroprotective effect in DG. On the contrary, carbonylation level of dihydropyrimidinase related protein 2 (DRP-2) and l-lactate dehydrogenase B chain (LDHB) were slightly increased in CA1 as shown previously, but remarkably increased in DG after ischemia. It is considered that DRP-2 and LDHB are specific targets of oxidative stress by ischemia insult and high carbonylation levels of DRP-2 may play an important role in modulating ischemic neuronal death. |
| تدمد: | 1880-5086 0912-0009 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7fceb3f2fe4702ce5f3ec78357f08a76 https://doi.org/10.3164/jcbn.19-78 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....7fceb3f2fe4702ce5f3ec78357f08a76 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18805086 09120009 |
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