Correlative cryo-electron microscopy reveals the structure of TNTs in neuronal cells
| العنوان: | Correlative cryo-electron microscopy reveals the structure of TNTs in neuronal cells |
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| المؤلفون: | Anna Sartori-Rupp, Jacomina Krijnse-Locker, Anna Pepe, Diégo Cordero Cervantes, Elise Delage, Chiara Zurzolo, Karine Gousset, Christine Schmitt, Simon Corroyer-Dulmont |
| المساهمون: | Plateforme BioImagerie Ultrastructurale – Ultrastructural BioImaging Platform (UTechS UBI), Institut Pasteur [Paris] (IP), Trafic membranaire et Pathogénèse, California State University [Fresno] (Fresno State), We acknowledge the Ultrastructural Bio-Imaging facility at Institut Pasteur, member of the national infrastructure France-Bio-Imaging (FBI) supported by the French National Research Agency (ANR-10-INBS-04) and IBISA.This work was supported by grants from the Agence Nationale de Recherche (JPND Neutargets: ANR-14-JPCD-0002-01 and ANR-16 CE160019-01 NEUROTUNN), and the Equipe FRM (Fondation Recherche Médicale) 2014 (DEQ20140329557) to C.Z. D.C.Cwas supported by the Pasteur—Paris University (PPU) International PhD Program. A.P.was supported by fellowships from France Parkinson and the Fondazione ErmenegildoZegna., We thank Seng Zhu for image analysis technical expertize and Christel Brou for valuable manuscript comments. We also gratefully acknowledge Gerard Péhau-Arnaudet (Ultrapole, Institut Pasteur) for his help in setting up freezing conditions of CAD cells during early stages of the project, and Remi Blanc from Amira (FEI, Thermo Fischer Scientific) for the automated segmentation of actin in Fig. 6 using the XTracing—Filament detection module. We thank the equipment excellence CACSICE for providing the Falcon II direct electron detector, ANR-10-INBS-0004,France-BioImaging,Développment d'une infrastructure française distribuée coordonnée(2010), ANR-14-JPCD-0002,Neutargets,Targeting the propagation of pathogenic protein assemblies in neurodegenerative disease(2014), ANR-16-CE16-0019,Neurotunn,Role des nanotubes membranaires dans la propagation d'agrégats protéiques impliqués dans les maladie neurodégénératives(2016), Sartori-Rupp, A., Cordero Cervantes, D., Pepe, A., Gousset, K., Delage, E., Corroyer-Dulmont, S., Schmitt, C., Krijnse-Locker, J., Zurzolo, C., Institut Pasteur [Paris] |
| المصدر: | Nature Communications Nature Communications, 2019, 10 (1), pp.342. ⟨10.1038/s41467-018-08178-7⟩ Nature Communications, Vol 10, Iss 1, Pp 1-16 (2019) Nature Communications, Nature Publishing Group, 2019, 10 (1), pp.342. ⟨10.1038/s41467-018-08178-7⟩ |
| بيانات النشر: | HAL CCSD, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Cryo-electron microscopy, Cell, MESH: Neurons, General Physics and Astronomy, 02 engineering and technology, Mice, Catecholamines, Microscopy, MESH: Animals, lcsh:Science, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, Neurons, Multidisciplinary, Cell Surface Extension, Chemistry, MESH: Cell Surface Extensions, 021001 nanoscience & nanotechnology, medicine.anatomical_structure, Catecholamine, MESH: Cryoelectron Microscopy, 0210 nano-technology, Human, Science, MESH: Biological Transport, Article, General Biochemistry, Genetics and Molecular Biology, Cell Line, 03 medical and health sciences, Organelle, medicine, MESH: Catecholamines, Animals, Humans, MESH: Mice, Actin, Organelles, MESH: Humans, Animal, Intercellular transport, Cryoelectron Microscopy, Biological Transport, General Chemistry, Neuron, MESH: Cell Line, Multicellular organism, 030104 developmental biology, Cytoplasm, Biophysics, lcsh:Q, Cell Surface Extensions, MESH: Organelles |
| الوصف: | The orchestration of intercellular communication is essential for multicellular organisms. One mechanism by which cells communicate is through long, actin-rich membranous protrusions called tunneling nanotubes (TNTs), which allow the intercellular transport of various cargoes, between the cytoplasm of distant cells in vitro and in vivo. With most studies failing to establish their structural identity and examine whether they are truly open-ended organelles, there is a need to study the anatomy of TNTs at the nanometer resolution. Here, we use correlative FIB-SEM, light- and cryo-electron microscopy approaches to elucidate the structural organization of neuronal TNTs. Our data indicate that they are composed of a bundle of open-ended individual tunneling nanotubes (iTNTs) that are held together by threads labeled with anti-N-Cadherin antibodies. iTNTs are filled with parallel actin bundles on which different membrane-bound compartments and mitochondria appear to transfer. These results provide evidence that neuronal TNTs have distinct structural features compared to other cell protrusions. The architecture of functional TNTs is still under debate. Here, the authors combine correlative FIB-SEM, light- and cryo-electron microscopy approaches to elucidate the structure of TNTs in neuronal cells, showing that they form structures that are distinct form other membrane protrusions. |
| اللغة: | English |
| تدمد: | 2041-1723 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::80f725053d5b098a05c94c9581fc51fa https://hal-pasteur.archives-ouvertes.fr/pasteur-02263612/document |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....80f725053d5b098a05c94c9581fc51fa |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20411723 |
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