Atypical structural snapshots of human cytomegalovirus GPCR interactions with host G proteins
| العنوان: | Atypical structural snapshots of human cytomegalovirus GPCR interactions with host G proteins |
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| المؤلفون: | Naotaka Tsutsumi, Shoji Maeda, Qianhui Qu, Martin Vögele, Kevin M. Jude, Carl-Mikael Suomivuori, Ouliana Panova, Deepa Waghray, Hideaki E. Kato, Andrew Velasco, Ron O. Dror, Georgios Skiniotis, Brian K. Kobilka, K. Christopher Garcia |
| المصدر: | Science Advances |
| بيانات النشر: | American Association for the Advancement of Science (AAAS), 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Mammals, Multidisciplinary, Cryoelectron Microscopy, SciAdv r-articles, Cytomegalovirus, Viral Proteins, Structural Biology, GTP-Binding Proteins, Virology, Animals, Humans, Receptors, Chemokine, Biomedicine and Life Sciences, Health and Medicine, Research Article |
| الوصف: | Human cytomegalovirus (HCMV) encodes G protein–coupled receptors (GPCRs) US28 and US27, which facilitate viral pathogenesis through engagement of host G proteins. Here we report cryo–electron microscopy structures of US28 and US27 forming nonproductive and productive complexes with Gi and Gq, respectively, exhibiting unusual features with functional implications. The “orphan” GPCR US27 lacks a ligand-binding pocket and has captured a guanosine diphosphate–bound inactive Gi through a tenuous interaction. The docking modes of CX3CL1-US28 and US27 to Gi favor localization to endosome-like curved membranes, where US28 and US27 can function as nonproductive Gi sinks to attenuate host chemokine-dependent Gi signaling. The CX3CL1-US28-Gq/11 complex likely represents a trapped intermediate during productive signaling, providing a view of a transition state in GPCR–G protein coupling for signaling. Our collective results shed new insight into unique G protein–mediated HCMV GPCR structural mechanisms, compared to mammalian GPCR counterparts, for subversion of host immunity. Description The HCMV GPCR-human G protein structures reveal unusual host-pathogen interactions likely critical for HCMV’s pathogenesis. |
| تدمد: | 2375-2548 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8100dc08e57e41c6a08ab1d8d2caba19 https://doi.org/10.1126/sciadv.abl5442 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....8100dc08e57e41c6a08ab1d8d2caba19 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23752548 |
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