Identifying Chloris Species from Cuban Citrus Orchards and Determining Their Glyphosate-Resistance Status
| العنوان: | Identifying Chloris Species from Cuban Citrus Orchards and Determining Their Glyphosate-Resistance Status |
|---|---|
| المؤلفون: | Fernando Bastida, Enzo Bracamonte, Rafael De Prado, Pablo Tomás Fernández-Moreno, Ricardo Alcántara-de la Cruz, Maria D. Osuna, Hugo E. Cruz-Hipolito |
| المصدر: | Arias Montano. Repositorio Institucional de la Universidad de Huelva instname Frontiers in Plant Science LOCUS Repositório Institucional da UFV Universidade Federal de Viçosa (UFV) instacron:UFV Frontiers in Plant Science, Vol 8 (2017) |
| بيانات النشر: | University of Bern, Switzerland, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Population, 5-enolpyruvyl, Chromosomal translocation, Subtropics, Plant Science, lcsh:Plant culture, Biology, tall windmill grass, Photosynthesis, 01 natural sciences, Glyphosate translocation, glyphosate translocation, 03 medical and health sciences, chemistry.chemical_compound, Herbicide resistance mechanisms, Botany, lcsh:SB1-1110, education, herbicide resistance mechanisms, Original Research, education.field_of_study, Pro-106 mutation, Ciliata, Tall windmill grass, Shikimic acid, Weed control, biology.organism_classification, 030104 developmental biology, chemistry, Glyphosate, 5-enolpyruvyl shikimate-3-phosphate synthase, 010606 plant biology & botany, Shikimate-3-phosphate synthase |
| الوصف: | The Chloris genus is a C4 photosynthetic species mainly distributed in tropical and subtropical regions. Populations of three Chloris species occurring in citrus orchards from central Cuba, under long history glyphosate-based weed management, were studied for glyphosate-resistant status by characterizing their herbicide resistance/tolerance mechanisms. Morphological and molecular analyses allowed these species to be identified as C. ciliata Sw., Chloris elata Desv., and Chloris barbata Sw. Based on the glyphosate rate that causes 50%mortality of the treated plants, glyphosate resistance (R) was confirmed only in C. elata, The R population was 6.1-fold more resistant compared to the susceptible (S) population. In addition, R plants of C. elata accumulated 4.6-fold less shikimate after glyphosate application than S plants. Meanwhile, populations of C. barbata and C. ciliata with or without glyphosate application histories showed similar LD50 values and shikimic acid accumulation rates, demonstrating that resistance to glyphosate have not evolved in these species. Plants of R and S populations of C. elata differed in 14C-glyphosate absorption and translocation. The R population exhibited 27.3-fold greater 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) activity than the S population due to a target site mutation corresponding to a Pro-106-Ser substitution found in the EPSPS gene. These reports show the innate tolerance to glyphosate of C. barbata and C. ciliata, and confirm the resistance of C. elata to this herbicide, showing that both non-target site and target-site mechanisms are involved in its resistance to glyphosate. This is the first case of herbicide resistance in Cuba. This work was funded by the AGL2016-78944-R (MICINN, Spain), GR15112 (Research Group AGA001 - Junta de Extremadura, Spain) and 242088 (CONACYT, Mexico) projects. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::812c8d22b371c0cda203f1184329d650 http://hdl.handle.net/10272/16086 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....812c8d22b371c0cda203f1184329d650 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |