Transcriptomics of receptive endometrium in women with sonographic features of adenomyosis
| العنوان: | Transcriptomics of receptive endometrium in women with sonographic features of adenomyosis |
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| المؤلفون: | Erika Prašnikar, Tanja Kunej, Mario Gorenjak, Uroš Potočnik, Borut Kovačič, Jure Knez |
| المصدر: | Reproductive Biology and Endocrinology : RB&E Reproductive Biology and Endocrinology, Vol 20, Iss 1, Pp 1-16 (2022) Reproductive biology and endocrinology, vol. 20, no. 2, 2022. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Adult, QH471-489, endometrial receptivity, Slovenia, Assisted reproductive techniques (ART), udc:616:575, enrichment pathway analysis, Enrichment pathway analysis, Omics approaches, transcriptomics, Endometrium, Endocrinology, Pregnancy, Humans, Embryo Implantation, Transcriptomics, data integration, Window of implantation, Ultrasonography, omics approaches, Reproduction, Gene Expression Profiling, Research, Obstetrics and Gynecology, systems biology, adenomioza, Gynecology and obstetrics, assisted reproductive techniques (ART), Reproductive Medicine, genetika, Endometrial receptivity, adenomyosis, window of implantation, Case-Control Studies, RG1-991, Female, Data integration, RNA-seq, Transcriptome, Systems biology, medicina, Adenomyosis, Developmental Biology |
| الوصف: | Background Women with uterine adenomyosis seeking assisted reproduction have been associated with compromised endometrial receptivity to embryo implantation. To understand the mechanisms involved in this process, we aimed to compare endometrial transcriptome profiles during the window of implantation (WOI) between women with and without adenomyosis. Methods We obtained endometrial biopsies LH-timed to the WOI from women with sonographic features of adenomyosis (n=10) and controls (n=10). Isolated RNA samples were subjected to RNA sequencing (RNA-seq) by the Illumina NovaSeq 6000 platform and endometrial receptivity classification with a molecular tool for menstrual cycle phase dating (beREADY®, CCHT). The program language R and Bioconductor packages were applied to analyse RNA-seq data in the setting of the result of accurate endometrial dating. To suggest robust candidate pathways, the identified differentially expressed genes (DEGs) associated with the adenomyosis group in the receptive phase were further integrated with 151, 173 and 42 extracted genes from published studies that were related to endometrial receptivity in healthy uterus, endometriosis and adenomyosis, respectively. Enrichment analyses were performed using Cytoscape ClueGO and CluePedia apps. Results Out of 20 endometrial samples, 2 were dated to the early receptive phase, 13 to the receptive phase and 5 to the late receptive phase. Comparison of the transcriptomics data from all 20 samples provided 909 DEGs (p Conclusions Accurate endometrial dating and RNA-seq analysis resulted in the identification of altered response to IFN signalling as the most promising candidate of impaired uterine receptivity in adenomyosis. |
| وصف الملف: | application/pdf; text/url |
| تدمد: | 1477-7827 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::813149cb59e4dc214ac10cd825c0fb51 https://pubmed.ncbi.nlm.nih.gov/34980152 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....813149cb59e4dc214ac10cd825c0fb51 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14777827 |
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