Intracellular modifications induced by poliovirus reduce the requirement for structural motifs in the 5' noncoding region of the genome involved in internal initiation of protein synthesis
| العنوان: | Intracellular modifications induced by poliovirus reduce the requirement for structural motifs in the 5' noncoding region of the genome involved in internal initiation of protein synthesis |
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| المؤلفون: | Graham J. Belsham, Julia K. Brangwyn, N Percy, M Sullivan, Jeffrey W. Almond, David M. Stone |
| المصدر: | Journal of Virology. 66:1695-1701 |
| بيانات النشر: | American Society for Microbiology, 1992. |
| سنة النشر: | 1992 |
| مصطلحات موضوعية: | Gene Expression Regulation, Viral, viruses, DNA Mutational Analysis, Immunology, Biology, medicine.disease_cause, Microbiology, Ribosome, Viral Proteins, Eukaryotic translation, Virology, Protein biosynthesis, medicine, RNA, Messenger, Genetics, Molecular Structure, Poliovirus, RNA, Hydrogen Bonding, Translation (biology), Open reading frame, Internal ribosome entry site, Protein Biosynthesis, Insect Science, RNA, Viral, Ribosomes, Plasmids, Research Article |
| الوصف: | A series of genetic deletions based partly on two RNA secondary structure models (M. A. Skinner, V. R. Racaniello, G. Dunn, J. Cooper, P. D. Minor, and J. W. Almond, J. Mol. Biol. 207:379-392, 1989; E. V. Pilipenko, V. M. Blinov, L. I. Romanova, A. N. Sinyakov, S. V. Maslova, and V. I. Agol, Virology 168:201-209, 1989) was made in the cDNA encoding the 5' noncoding region (5' NCR) of the poliovirus genome in order to study the sequences that direct the internal entry of ribosomes. The modified cDNAs were placed between two open reading frames in a single transcriptional unit and used to transfect cells in culture. Internal entry of ribosomes was detected by measuring translation from the second open reading frame in the bicistronic mRNA. When assayed alone, a large proportion of the poliovirus 5' NCR superstructure including several well-defined stem-loops was required for ribosome entry and efficient translation. However, in cells cotransfected with a complete infectious poliovirus cDNA, the requirement for the stem-loops in this large superstructure was reduced. The results suggest that virus infection modifies the cellular translational machinery, so that shortened forms of the 5' NCR are sufficient for cap-independent translation, and that the internal entry of ribosomes occurs by two distinct modes during the virus replication cycle. |
| تدمد: | 1098-5514 0022-538X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::81af0d0b9526f28adaa1634090384fea https://doi.org/10.1128/jvi.66.3.1695-1701.1992 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....81af0d0b9526f28adaa1634090384fea |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985514 0022538X |
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