Light-assisted small-molecule screening against protein kinases
| العنوان: | Light-assisted small-molecule screening against protein kinases |
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| المؤلفون: | Harald Janovjak, Matthias Nowak, Markus K Muellner, Sebastian M.B. Nijman, Eva Reichhart, Alvaro Ingles-Prieto, Michael Grusch |
| المصدر: | Nature chemical biology |
| بيانات النشر: | Springer Science and Business Media LLC, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Orphan receptor, Light, Drug discovery, Phenotypic screening, Systems biology, Small Molecule Libraries, Cell Biology, Biology, Small molecule, Article, High-Throughput Screening Assays, Cell biology, Structure-Activity Relationship, HEK293 Cells, High-content screening, 571 Physiology, Humans, Protein Kinase Inhibitors, Protein Kinases, Molecular Biology, Tyrosine kinase |
| الوصف: | High-throughput live-cell screens are intricate elements of systems biology studies and drug discovery pipelines. Here, we demonstrate an optogenetics-assisted method that obviates the addition of chemical activators and reporters, reduces the number of operational steps and increases information content in a cell-based small molecule screen against human protein kinases including an orphan receptor tyrosine kinase. This blueprint for all-optical screening can be adapted to many drug targets and cellular processes. |
| وصف الملف: | text |
| تدمد: | 1552-4469 1552-4450 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::821779cc71bd7420a0c16b5693764e10 https://doi.org/10.1038/nchembio.1933 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....821779cc71bd7420a0c16b5693764e10 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15524469 15524450 |
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