Purification of mammalian tubulins and tubulin-associated proteins using a P2A-based expression system
| العنوان: | Purification of mammalian tubulins and tubulin-associated proteins using a P2A-based expression system |
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| المؤلفون: | Yu, Nuo, Galjart, Niels, Maiato, H. |
| المساهمون: | Cell biology |
| المصدر: | Cytoskeleton Dynamics : methods and protocols, 2101, 1-17 Methods in Molecular Biology ISBN: 9781071602188 |
| بيانات النشر: | Humana Press Inc, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, biology, Chemistry, Microtubule cytoskeleton, Cellular differentiation, macromolecular substances, Cell biology, 03 medical and health sciences, chemistry.chemical_compound, 030104 developmental biology, 0302 clinical medicine, Tubulin, Biosynthesis, Microtubule, biology.protein, Cell shape, Mitosis, 030217 neurology & neurosurgery, Function (biology) |
| الوصف: | The microtubule cytoskeleton plays a crucial role in a myriad of cellular events, including mitosis, cell differentiation, migration, and the maintenance of cell shape. Microtubules are assembled from α- and β-tubulin heterodimers, whose biosynthesis is a complex process requiring the balanced production of α- and β-tubulin subunits. This chapter focuses on a method for the combined expression of tagged α- and β-tubulin dimers, their purification, and the isolation of co-purifying tubulin-associated proteins (TAPs) in mammalian cells. This approach is currently used in our laboratory to study tubulin function and to identify and characterize TAPs. |
| اللغة: | English |
| ردمك: | 978-1-07-160218-8 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8277ba1a36b40c135a005b77be655684 https://pure.eur.nl/en/publications/091fd246-9a1f-489f-8d38-04764c32c74c |
| حقوق: | RESTRICTED |
| رقم الأكسشن: | edsair.doi.dedup.....8277ba1a36b40c135a005b77be655684 |
| قاعدة البيانات: | OpenAIRE |
| ردمك: | 9781071602188 |
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