An investigation of aspects ranging from behavior to molecular electronic structure and physicochemical properties was performed to explore the role of 5α-pregnanedione (5α-DHP), 5β-pregnanedione (5β-DHP) and their precursor progesterone (P) on the concurrent inhibition of the sexual lordosis response in female rats. The concurrent inhibition of lordosis behavior occurs when ovariectomized rodents are primed simultaneously with estradiol (E2) and P. Thus, a second administration of P 40h later fails to induce the expected sexual response that takes place when E2 and P are administered sequentially 40h apart. In this study, it is hypothesized that the modulation of the sexual behavior display depends to some extent on the molecular structure and associated physicochemical properties of steroid hormones such as P and its metabolites. Therefore, these molecules must be studied chemically and structurally to explain their role in sexual behavior, including the concurrent inhibition effect. Analysis of the electronic structure and physicochemical properties demonstrated striking differences in the A-ring region of P, 5α-DHP and 5β-DHP, particularly in atomic charges, dipole moment (DM) and electrostatic potentials. Similarly, the structural differences between the trans (5α-DHP) and cis (5β-DHP) configurations were remarkable. 5α-DHP most significantly promoted the concurrent inhibition of the lordosis behavior, followed by P and 5β-DHP. These data indicate that variations in pregnane structure are related to the extent of the concurrent inhibition effect and also suggest that P may act as a prehormone in certain functions of the central nervous system.
