Ablation of KDM2A Inhibits Preadipocyte Proliferation and Promotes Adipogenic Differentiation
| العنوان: | Ablation of KDM2A Inhibits Preadipocyte Proliferation and Promotes Adipogenic Differentiation |
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| المؤلفون: | Dan Zhao, Yan Xiong, Yan Ma, Yongqi Yue, Xianrong Xiong, Jian Li, Yonglin Hua |
| المصدر: | International Journal of Molecular Sciences, Vol 22, Iss 9759, p 9759 (2021) International Journal of Molecular Sciences Volume 22 Issue 18 |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | preadipocytes, Small interfering RNA, Jumonji Domain-Containing Histone Demethylases, PPARγ, QH301-705.5, Adipose Tissue, White, proliferation, KDM2A, Catalysis, Article, Inorganic Chemistry, Gene Knockout Techniques, Mice, Lipid droplet, 3T3-L1 Cells, Adipocytes, Animals, Epigenetics, Physical and Theoretical Chemistry, Biology (General), Molecular Biology, TNPO1, QD1-999, Spectroscopy, Cell Proliferation, Gene knockdown, Adipogenesis, biology, Chemistry, Organic Chemistry, Cell Differentiation, General Medicine, differentiation, Computer Science Applications, Cell biology, Protein Transport, Gene Expression Regulation, Transportin 1, biology.protein, fat deposition, Demethylase |
| الوصف: | Epigenetic signals and chromatin-modifying proteins play critical roles in adipogenesis, which determines the risk of obesity and which has recently attracted increasing interest. Histone demethylase 2A (KDM2A) is an important component of histone demethylase however, its direct effect on fat deposition remains unclear. Here, a KDM2A loss of function was performed using two unbiased methods, small interfering RNA (siRNA) and Cre-Loxp recombinase systems, to reveal its function in adipogenesis. The results show that the knockdown of KDM2A by siRNAs inhibited the proliferation capacity of 3T3-L1 preadipocytes. Furthermore, the promotion of preadipocyte differentiation was observed in siRNA-treated cells, manifested by the increasing content of lipid droplets and the expression level of adipogenic-related genes. Consistently, the genetic deletion of KDM2A by Adipoq-Cre in primary adipocytes exhibited similar phenotypes to those of 3T3-L1 preadipocytes. Interestingly, the knockdown of KDM2A upregulates the expression level of Transportin 1(TNPO1), which in turn may induce the nuclear translocation of PPARγ and the accumulation of lipid droplets. In conclusion, the ablation of KDM2A inhibits preadipocyte proliferation and promotes its adipogenic differentiation. This work provides direct evidence of the exact role of KDM2A in fat deposition and provides theoretical support for obesity therapy that targets KDM2A. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1661-6596 1422-0067 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::82b040d590419d99dfc8f4e00563cce8 https://www.mdpi.com/1422-0067/22/18/9759 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....82b040d590419d99dfc8f4e00563cce8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16616596 14220067 |
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