Polyacrylamide gel electrophoresis of isoenzymes from Entamoeba species
| العنوان: | Polyacrylamide gel electrophoresis of isoenzymes from Entamoeba species |
|---|---|
| المؤلفون: | Delynn M. Moss, G. R. Healy, H M Mathews, Govinda S. Visvesvara |
| المصدر: | Journal of Clinical Microbiology. 17:1009-1012 |
| بيانات النشر: | American Society for Microbiology, 1983. |
| سنة النشر: | 1983 |
| مصطلحات موضوعية: | Microbiology (medical), biology, Entamoeba histolytica, Glucose-6-Phosphate Isomerase, Entamoeba, Entamoeba coli, biology.organism_classification, Malate dehydrogenase, Isozyme, Molecular biology, Isoenzymes, Electrophoresis, Phosphoglucomutase, Biochemistry, Malate Dehydrogenase, Hexokinase, parasitic diseases, Electrophoresis, Polyacrylamide Gel, Polyacrylamide gel electrophoresis, Research Article |
| الوصف: | In this preliminary report, we describe a polyacrylamide gel electrophoresis technique for the resolution of isoenzyme patterns of four isolates of Entamoeba histolytica and one isolate of Entamoeba coli. Our findings were similar to previous findings for three enzyme systems: maleic enzyme (malate dehydrogenase [EC 1.1.1.40]), hexokinase (EC 2.7.1.1), and phosphoglucomutase (EC 2.7.5.1). We found preliminary evidence that glucosephosphate isomerase (EC 5.3.1.9) may also differentiate invasive amoebae from noninvasive amoebae, when the isoenzymes are separated by polyacrylamide gel electrophoresis, whereas this differentiation is not evident with starch-gel electrophoresis. We used an Rf system to relate isoenzyme band mobility to the migration distance of a standard E. histolytica strain (HK-9). The numerical identification of isoenzyme bands can simplify the grouping of isolates into zymodemes. |
| تدمد: | 1098-660X 0095-1137 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::82c9c3605e0acac3fffdfd96eb0250de https://doi.org/10.1128/jcm.17.6.1009-1012.1983 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....82c9c3605e0acac3fffdfd96eb0250de |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1098660X 00951137 |
|---|