Influenza A virus utilizes noncanonical cap-snatching to diversify its mRNA/ncRNA
| العنوان: | Influenza A virus utilizes noncanonical cap-snatching to diversify its mRNA/ncRNA |
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| المؤلفون: | Lichao Li, Weifeng Gu, An-phong Nguyen, Hui Dai, Rong Hai |
| المصدر: | RNA (New York, N.Y.), vol 26, iss 9 RNA |
| بيانات النشر: | Cold Spring Harbor Laboratory, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | MRNA synthesis, RNA, Untranslated, Transcription, Genetic, Messenger, novel influenza virus mRNA, Priming (immunology), priming and realignment, medicine.disease_cause, influenza virus, Influenza A virus, Nucleotide, Viral, Base Pairing, chemistry.chemical_classification, 0303 health sciences, Tumor, 030302 biochemistry & molecular biology, Untranslated, noncoding RNA or ncRNA, Non-coding RNA, Cell biology, Infectious Diseases, Pneumonia & Influenza, RNA, Viral, Transcription, RNA Caps, Biology, Article, Cell Line, Cap snatching, Vaccine Related, 03 medical and health sciences, Genetic, Cell Line, Tumor, Biodefense, Genetics, medicine, Humans, RNA, Messenger, Molecular Biology, 030304 developmental biology, Messenger RNA, Prevention, cap-snatching, RNA-Dependent RNA Polymerase, Influenza, Emerging Infectious Diseases, chemistry, A549 Cells, RNA, Biochemistry and Cell Biology, Developmental Biology |
| الوصف: | Influenza A virus (IAV) utilizes cap-snatching to obtain host capped small RNAs for priming viral mRNA synthesis, generating capped hybrid mRNAs for translation. Previous studies have been focusing on canonical cap-snatching, which occurs at the very 5′ end of viral mRNAs. Here we discovered noncanonical cap-snatching, which generates capped hybrid mRNAs/noncoding RNAs mapped to the region ∼300 nucleotides (nt) upstream of each mRNA 3′ end, and to the 5′ region, primarily starting at the second nt, of each virion RNAs (vRNA). Like canonical cap-snatching, noncanonical cap-snatching utilizes a base-pairing between the last nt G of host capped RNAs and a nt C of template RNAs to prime RNA synthesis. However, the nt upstream of this template C is usually A/U rather than just U; prime-realignment occurs less frequently. We also demonstrate that IAV can snatch capped IAV RNAs in addition to host RNAs. Noncanonical cap-snatching likely generates novel mRNAs with start AUG encoded in viral or host RNAs. These findings expand our understanding of cap-snatching mechanisms and suggest that IAV may utilize noncanonical cap-snatching to diversify its mRNAs/ncRNAs. |
| وصف الملف: | application/pdf |
| تدمد: | 1469-9001 1355-8382 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::82e906340cfe2f7f7bad27b3d04cf027 https://doi.org/10.1261/rna.073866.119 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....82e906340cfe2f7f7bad27b3d04cf027 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14699001 13558382 |
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