The cytotoxicity of eosinophil cationic protein/ribonuclease 3 on eukaryotic cell lines takes place through its aggregation on the cell membrane
| العنوان: | The cytotoxicity of eosinophil cationic protein/ribonuclease 3 on eukaryotic cell lines takes place through its aggregation on the cell membrane |
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| المؤلفون: | Marcel Jiménez, Claudi M. Cuchillo, Ester Boix, Jordi Aleu, M.V. Nogués, Susanna Navarro |
| المصدر: | Cellular and Molecular Life Sciences. 65:324-337 |
| بيانات النشر: | Springer Science and Business Media LLC, 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Programmed cell death, Cell Membrane Permeability, Time Factors, RNase P, media_common.quotation_subject, Lipid Bilayers, education, Cell, Intracellular Space, HL-60 Cells, Phosphatidylserines, Protein aggregation, Cell Line, Cell membrane, Cellular and Molecular Neuroscience, fluids and secretions, medicine, Humans, Ribonuclease, Protein Structure, Quaternary, Internalization, Molecular Biology, media_common, Pharmacology, Eosinophil cationic protein, Microscopy, Confocal, Cell Death, biology, Cell Cycle, Cell Membrane, Eosinophil Cationic Protein, Cell Biology, Caspase Inhibitors, Cell biology, Protein Transport, Eukaryotic Cells, medicine.anatomical_structure, biology.protein, RNA, Molecular Medicine, Calcium, Reactive Oxygen Species, HeLa Cells |
| الوصف: | Human eosinophil cationic protein (ECP)/ ribonuclease 3 (RNase 3) is a protein secreted from the secondary granules of activated eosinophils. Specific properties of ECP contribute to its cytotoxic activities associated with defense mechanisms. In this work the ECP cytotoxic activity on eukaryotic cell lines is analyzed. The ECP effects begin with its binding and aggregation to the cell surface, altering the cell membrane permeability and modifying the cell ionic equilibrium. No internalization of the protein is observed. These signals induce cell-specific morphological and biochemical changes such as chromatin condensation, reversion of membrane asymmetry, reactive oxygen species production and activation of caspase-3-like activity and, eventually, cell death. However, the ribonuclease activity component of ECP is not involved in this process as no RNA degradation is observed. In summary, the cytotoxic effect of ECP is attained through a mechanism different from that of other cytotoxic RNases and may be related with the ECP accumulation associated with the inflammatory processes, in which eosinophils are present. |
| تدمد: | 1420-9071 1420-682X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::82ea139da05084b050f195a6c89d6ee4 https://doi.org/10.1007/s00018-007-7499-7 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....82ea139da05084b050f195a6c89d6ee4 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14209071 1420682X |
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