Simultaneous Determination of Chlorogenic Acid Isomers and Metabolites in Rat Plasma Using LC-MS/MS and Its Application to A Pharmacokinetic Study Following Oral Administration of Stauntonia Hexaphylla Leaf Extract (YRA-1909) to Rats
| العنوان: | Simultaneous Determination of Chlorogenic Acid Isomers and Metabolites in Rat Plasma Using LC-MS/MS and Its Application to A Pharmacokinetic Study Following Oral Administration of Stauntonia Hexaphylla Leaf Extract (YRA-1909) to Rats |
|---|---|
| المؤلفون: | Dong Kyun Kim, Ju-Hyun Kim, Hye Suk Lee, Yongnam Lee, Ji Seok Yoo, Dae Hee Shin, Won-Gu Choi |
| المصدر: | Pharmaceutics, Vol 10, Iss 3, p 143 (2018) Pharmaceutics Volume 10 Issue 3 |
| بيانات النشر: | MDPI AG, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Formic acid, chlorogenic acid, Ethyl acetate, Pharmaceutical Science, caffeic acid O-glucuronides, lcsh:RS1-441, 030226 pharmacology & pharmacy, 01 natural sciences, Ferulic acid, lcsh:Pharmacy and materia medica, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Chlorogenic acid, Pharmacokinetics, Caffeic acid, LC-MS/MS, neochlorogenic acid, Stauntonia hexaphylla leaf extract, cryptochlorogenic acid, Neochlorogenic acid, Chromatography, 010401 analytical chemistry, Selected reaction monitoring, YRA-1909, 0104 chemical sciences, chemistry, caffeic acid, pharmacokinetics |
| الوصف: | Stauntonia hexaphylla leaf extract (YRA-1909), which is widely used for the antirheumatic properties, has been under phase 2 clinical trials in patients with rheumatoid arthritis since April 2017. Liquid chromatography-tandem mass spectrometric method while using liquid&ndash liquid extraction with ethyl acetate was validated for the simultaneous determination of the major active components of YRA-1909, including chlorogenic acid (CGA), neochlorogenic acid (NCGA), cryptochlorogenic acid (CCGA), and their metabolites (i.e., caffeic acid (CA), caffeic acid 3-O-glucuronide (CA-3-G), caffeic acid 4-O-glucuronide (CA-4-G), and ferulic acid (FA)) in rat plasma and applied to a pharmacokinetic study of YRA-1909 in rats. Seven analytes were separated on Halo C18 while using gradient elution of formic acid and methanol, and then quantified in selected reaction monitoring mode whle using negative electrospray ionization. Following oral administration of YRA-1909 at doses of 25, 50, and 100 mg/kg to male Sprague-Dawley rats, CGA, NCGA, and CCGA were rapidly absorbed and metabolized to CA, CA-3-G, and CA-4-G. The area under the plasma concentration-time curve (AUClast) of CGA, NCGA, CCGA, and three metabolites linearly increased as the YRA-1909 dose increased. Other pharmacokinetic parameters were comparable among three doses studied. AUClast values for CA, CA-3-G, and CA-4-G exceeded those for CGA, NCGA, and CCGA. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1999-4923 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::836183df4987cb94e8bf370d8b6f2e26 http://www.mdpi.com/1999-4923/10/3/143 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....836183df4987cb94e8bf370d8b6f2e26 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19994923 |
|---|