Confocal Analysis of Nuclear Lamina Behavior during Male Meiosis and Spermatogenesis in Drosophila melanogaster
| العنوان: | Confocal Analysis of Nuclear Lamina Behavior during Male Meiosis and Spermatogenesis in Drosophila melanogaster |
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| المؤلفون: | Silvia Bongiorni, Giorgio Prantera, Ilaria Iannetti, Loredana Guglielmi, Luca Proietti De Santis, Susanna Perconti, Chiara Evangelistella, Fabiana Fabbretti |
| المصدر: | PLoS ONE, Vol 11, Iss 3, p e0151231 (2016) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Physiology, Gene Expression, lcsh:Medicine, Biochemistry, 0302 clinical medicine, Reproductive Physiology, Animal Cells, Spermatocytes, Medicine and Health Sciences, Cell Cycle and Cell Division, lcsh:Science, Staining, Genetics, Microscopy, Confocal, Multidisciplinary, biology, Chromosome Biology, Drosophila Melanogaster, Animal Models, Spermatids, Specimen preparation and treatment, Lamins, Chromatin, Cell biology, Insects, Meiosis, medicine.anatomical_structure, Cell Processes, Nuclear lamina, Drosophila, Epigenetics, Cellular Types, Drosophila melanogaster, Research Article, Arthropoda, Nuclear Envelope, Research and Analysis Methods, 03 medical and health sciences, Model Organisms, medicine, Animals, Inner membrane, Spermatogenesis, Mitosis, Cell Nucleus, Nuclear Lamina, lcsh:R, Organisms, DAPI staining, Biology and Life Sciences, Proteins, Cell Biology, biology.organism_classification, Invertebrates, Sperm, Cytoskeletal Proteins, Cell nucleus, Germ Cells, 030104 developmental biology, Nuclear staining, lcsh:Q, 030217 neurology & neurosurgery, Lamin |
| الوصف: | Lamin family proteins are structural components of a filamentous framework, the nuclear lamina (NL), underlying the inner membrane of nuclear envelope. The NL not only plays a role in nucleus mechanical support and nuclear shaping, but is also involved in many cellular processes including DNA replication, gene expression and chromatin positioning. Spermatogenesis is a very complex differentiation process in which each stage is characterized by nuclear architecture dramatic changes, from the early mitotic stage to the sperm differentiation final stage. Nevertheless, very few data are present in the literature on the NL behavior during this process. Here we show the first and complete description of NL behavior during meiosis and spermatogenesis in Drosophila melanogaster. By confocal imaging, we characterized the NL modifications from mitotic stages, through meiotic divisions to sperm differentiation with an anti-laminDm0 antibody against the major component of the Drosophila NL. We observed that continuous changes in the NL structure occurred in parallel with chromatin reorganization throughout the whole process and that meiotic divisions occurred in a closed context. Finally, we analyzed NL in solofuso meiotic mutant, where chromatin segregation is severely affected, and found the strict correlation between the presence of chromatin and that of NL. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8399c56c627b58f75985e0b6d4ba4717 http://europepmc.org/articles/PMC4786128?pdf=render |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....8399c56c627b58f75985e0b6d4ba4717 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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