High tumour mutational burden and EGFR/MAPK pathway activation are therapeutic targets in metastatic porocarcinoma*
| العنوان: | High tumour mutational burden and EGFR/MAPK pathway activation are therapeutic targets in metastatic porocarcinoma* |
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| المؤلفون: | A. Stenzinger, S. Ugurel, Stefan Fröhling, Sophia Blum, S Grosche-Schlee, Stefan Beissert, Daniela Aust, Marlene Garzarolli, Gustavo B. Baretton, Friedegund Meier, Silke Redler, M. Sergon, Dana Westphal, Jürgen C. Becker, Barbara Hutter, Arno Rütten, Harald Surowy, M Wiegel, E Maczey, Peter Horak, Hanno Glimm |
| المصدر: | British Journal of Dermatology. 185:1186-1199 |
| بيانات النشر: | Oxford University Press (OUP), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | MAPK/ERK pathway, biology, MAP Kinase Signaling System, business.industry, CTNND1, Kinase, Medizin, Dermatology, Eccrine Porocarcinoma, Cell cycle, Cyclin-Dependent Kinase Inhibitor 2A, ErbB Receptors, Sweat Gland Neoplasms, MAP2K1, Mutation, Cancer research, biology.protein, Humans, Medicine, Molecular Targeted Therapy, Epidermal growth factor receptor, Protein kinase A, business |
| الوصف: | Background: Eccrine porocarcinoma (EPC) is a rare skin cancer arising from the eccrine sweat glands. Due to the lack of effective therapies, metastasis is associated with a high mortality rate. Objectives: To investigate the drivers of EPC progression. Methods: We carried out genomic and transcriptomic profiling of metastatic EPC (mEPC), validation of the observed alterations in an EPC patient-derived cell line, confirmation of relevant observations in a large patient cohort of 30 tumour tissues, and successful treatment of a patient with mEPC under the identified treatment regimens. Results: mEPC was characterized by a high tumour mutational burden (TMB) with an ultraviolet signature, widespread copy number alterations and gene expression changes that affected cancer-relevant cellular processes such as cell cycle regulation and proliferation, including a pathogenic TP53 (tumour protein 53) mutation, a copy number deletion in the CDKN2A (cyclin dependent kinase inhibitor 2A) region and a CTNND1/PAK1 [catenin delta 1/p21 (RAC1) activated kinase 1] gene fusion. The overexpression of EGFR (epidermal growth factor receptor), PAK1 and MAP2K1 (mitogen-activated protein kinase kinase 1; also known as MEK1) genes translated into strong protein expression and respective pathway activation in the tumour tissue. Furthermore, a patient-derived cell line was sensitive to EGFR and MEK inhibition, confirming the functional relevance of the pathway activation. Immunohistochemistry analyses in a large patient cohort showed the relevance of the observed changes to the pathogenesis of EPC. Our results indicate that mEPC should respond to immune or kinase inhibitor therapy. Indeed, the advanced disease of our index patient was controlled by EGFR-directed therapy and immune checkpoint inhibition for more than 2 years. Conclusions: Molecular profiling demonstrated high TMB and EGFR/MAPK pathway activation to be novel therapeutic targets in mEPC. |
| تدمد: | 1365-2133 0007-0963 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::83c46b3eb537c6aa909606b8715e7211 https://doi.org/10.1111/bjd.20604 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....83c46b3eb537c6aa909606b8715e7211 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13652133 00070963 |
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